The MicroRNA Landscapes Profiling Reveals Potential Signatures of Necrotizing Enterocolitis in Infants
The purpose of this study is to extract potential signatures involved in necrotizing enterocolitis (NEC). The data set GSE68054 was acquired from Gene Expression Omnibus repertory, and differentially expressed genes were screened between NEC and control group. After that, the target prediction analysis and functional enrichment analysis were carried out. Moreover, protein-protein interaction (PPI) and functional analyses of top 20 key genes in PPI network were undertaken. In addition, drug-gene interaction prediction analysis was also carried out to screen the key genes for NEC treatment. Totally, there were 16 differentially expressed microRNAs (DEmiRNAs; 2 upregulated and 14 downregulated miRNAs) between NEC and controls. Furthermore, 354 DEmiRNAs-targets interactions were predicted, including KDR-miR-200c-3p and YWHAG-miR-200a-3p. Several genes such as BDNF, KDR, YWHAG, YWHAE, and YWHAB acted as hub genes in PPI network and functional analysis implied that multiple genes, including YWHAG, YWHAE, and YWHAB, were strongly linked with positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway, whereas BDNF and KDR were markedly enriched in PI3K-Akt signaling pathway and neurotrophin signaling pathway, respectively. Interestingly, we found that KDR showed strong correlations with 16 drugs based on drug-gene interaction predictive analysis. YWHAG, YWHAE, YWHAB, BDNF, and KDR might serve as promising therapeutic targets for NEC management. In addition, miR-200a-3p was also possibly involved in the molecular mechanism of CNEC, presenting deeper insights into NEC diagnosis and treatment.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:27 |
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| Enthalten in: |
Journal of computational biology : a journal of computational molecular cell biology - 27(2020), 1 vom: 01. Jan., Seite 30-39 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhao, Jing [VerfasserIn] |
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| Links: |
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| Themen: |
Drug–gene interaction analysis |
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| Anmerkungen: |
Date Completed 26.04.2021 Date Revised 26.04.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1089/cmb.2019.0183 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM300009828 |
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| 245 | 1 | 4 | |a The MicroRNA Landscapes Profiling Reveals Potential Signatures of Necrotizing Enterocolitis in Infants |
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| 520 | |a The purpose of this study is to extract potential signatures involved in necrotizing enterocolitis (NEC). The data set GSE68054 was acquired from Gene Expression Omnibus repertory, and differentially expressed genes were screened between NEC and control group. After that, the target prediction analysis and functional enrichment analysis were carried out. Moreover, protein-protein interaction (PPI) and functional analyses of top 20 key genes in PPI network were undertaken. In addition, drug-gene interaction prediction analysis was also carried out to screen the key genes for NEC treatment. Totally, there were 16 differentially expressed microRNAs (DEmiRNAs; 2 upregulated and 14 downregulated miRNAs) between NEC and controls. Furthermore, 354 DEmiRNAs-targets interactions were predicted, including KDR-miR-200c-3p and YWHAG-miR-200a-3p. Several genes such as BDNF, KDR, YWHAG, YWHAE, and YWHAB acted as hub genes in PPI network and functional analysis implied that multiple genes, including YWHAG, YWHAE, and YWHAB, were strongly linked with positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway, whereas BDNF and KDR were markedly enriched in PI3K-Akt signaling pathway and neurotrophin signaling pathway, respectively. Interestingly, we found that KDR showed strong correlations with 16 drugs based on drug-gene interaction predictive analysis. YWHAG, YWHAE, YWHAB, BDNF, and KDR might serve as promising therapeutic targets for NEC management. In addition, miR-200a-3p was also possibly involved in the molecular mechanism of CNEC, presenting deeper insights into NEC diagnosis and treatment | ||
| 650 | 4 | |a Journal Article | |
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