Arabidopsis ALIX Regulates Stomatal Aperture and Turnover of Abscisic Acid Receptors
© 2019 American Society of Plant Biologists. All rights reserved..
The plant endosomal trafficking pathway controls the abundance of membrane-associated soluble proteins, as shown for abscisic acid (ABA) receptors of the PYRABACTIN RESISTANCE1/PYR1-LIKE/REGULATORY COMPONENTS OF ABA RECEPTORS (PYR/PYL/RCAR) family. ABA receptor targeting for vacuolar degradation occurs through the late endosome route and depends on FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING1 (FYVE1) and VACUOLAR PROTEIN SORTING23A (VPS23A), components of the ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT-I (ESCRT-I) complexes. FYVE1 and VPS23A interact with ALG-2 INTERACTING PROTEIN-X (ALIX), an ESCRT-III-associated protein, although the functional relevance of such interactions and their consequences in cargo sorting are unknown. In this study we show that Arabidopsis (Arabidopsis thaliana) ALIX directly binds to ABA receptors in late endosomes, promoting their degradation. Impaired ALIX function leads to altered endosomal localization and increased accumulation of ABA receptors. In line with this activity, partial loss-of-function alix-1 mutants display ABA hypersensitivity during growth and stomatal closure, unveiling a role for the ESCRT machinery in the control of water loss through stomata. ABA-hypersensitive responses are suppressed in alix-1 plants impaired in PYR/PYL/RCAR activity, in accordance with ALIX affecting ABA responses primarily by controlling ABA receptor stability. ALIX-1 mutant protein displays reduced interaction with VPS23A and ABA receptors, providing a molecular basis for ABA hypersensitivity in alix-1 mutants. Our findings unveil a negative feedback mechanism triggered by ABA that acts via ALIX to control the accumulation of specific PYR/PYL/RCAR receptors.
| Errataetall: |
CommentIn: Plant Cell. 2019 Oct;31(10):2291-2292. - PMID 31444311 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:31 |
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| Enthalten in: |
The Plant cell - 31(2019), 10 vom: 30. Okt., Seite 2411-2429 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
García-León, Marta [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 29.06.2020 Date Revised 29.02.2024 published: Print-Electronic CommentIn: Plant Cell. 2019 Oct;31(10):2291-2292. - PMID 31444311 Citation Status MEDLINE |
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| doi: |
10.1105/tpc.19.00399 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM299745759 |
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| 245 | 1 | 0 | |a Arabidopsis ALIX Regulates Stomatal Aperture and Turnover of Abscisic Acid Receptors |
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| 500 | |a Date Revised 29.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a CommentIn: Plant Cell. 2019 Oct;31(10):2291-2292. - PMID 31444311 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2019 American Society of Plant Biologists. All rights reserved. | ||
| 520 | |a The plant endosomal trafficking pathway controls the abundance of membrane-associated soluble proteins, as shown for abscisic acid (ABA) receptors of the PYRABACTIN RESISTANCE1/PYR1-LIKE/REGULATORY COMPONENTS OF ABA RECEPTORS (PYR/PYL/RCAR) family. ABA receptor targeting for vacuolar degradation occurs through the late endosome route and depends on FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING1 (FYVE1) and VACUOLAR PROTEIN SORTING23A (VPS23A), components of the ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT-I (ESCRT-I) complexes. FYVE1 and VPS23A interact with ALG-2 INTERACTING PROTEIN-X (ALIX), an ESCRT-III-associated protein, although the functional relevance of such interactions and their consequences in cargo sorting are unknown. In this study we show that Arabidopsis (Arabidopsis thaliana) ALIX directly binds to ABA receptors in late endosomes, promoting their degradation. Impaired ALIX function leads to altered endosomal localization and increased accumulation of ABA receptors. In line with this activity, partial loss-of-function alix-1 mutants display ABA hypersensitivity during growth and stomatal closure, unveiling a role for the ESCRT machinery in the control of water loss through stomata. ABA-hypersensitive responses are suppressed in alix-1 plants impaired in PYR/PYL/RCAR activity, in accordance with ALIX affecting ABA responses primarily by controlling ABA receptor stability. ALIX-1 mutant protein displays reduced interaction with VPS23A and ABA receptors, providing a molecular basis for ABA hypersensitivity in alix-1 mutants. Our findings unveil a negative feedback mechanism triggered by ABA that acts via ALIX to control the accumulation of specific PYR/PYL/RCAR receptors | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a ALIX protein, Arabidopsis |2 NLM | |
| 650 | 7 | |a Arabidopsis Proteins |2 NLM | |
| 650 | 7 | |a Carrier Proteins |2 NLM | |
| 650 | 7 | |a Endosomal Sorting Complexes Required for Transport |2 NLM | |
| 650 | 7 | |a Intracellular Signaling Peptides and Proteins |2 NLM | |
| 650 | 7 | |a Membrane Transport Proteins |2 NLM | |
| 650 | 7 | |a Plant Growth Regulators |2 NLM | |
| 650 | 7 | |a Receptors, Cell Surface |2 NLM | |
| 650 | 7 | |a Water |2 NLM | |
| 650 | 7 | |a 059QF0KO0R |2 NLM | |
| 650 | 7 | |a Abscisic Acid |2 NLM | |
| 650 | 7 | |a 72S9A8J5GW |2 NLM | |
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| 700 | 1 | |a Belda-Palazón, Borja |e verfasserin |4 aut | |
| 700 | 1 | |a Sanchez-Quant, Eva |e verfasserin |4 aut | |
| 700 | 1 | |a Fernández, Yolanda |e verfasserin |4 aut | |
| 700 | 1 | |a Roux, Brice |e verfasserin |4 aut | |
| 700 | 1 | |a Zamarreño, Ángel María |e verfasserin |4 aut | |
| 700 | 1 | |a García-Mina, José María |e verfasserin |4 aut | |
| 700 | 1 | |a Nussaume, Laurent |e verfasserin |4 aut | |
| 700 | 1 | |a Rodriguez, Pedro L |e verfasserin |4 aut | |
| 700 | 1 | |a Paz-Ares, Javier |e verfasserin |4 aut | |
| 700 | 1 | |a Leonhardt, Nathalie |e verfasserin |4 aut | |
| 700 | 1 | |a Rubio, Vicente |e verfasserin |4 aut | |
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