Nbseptin2 Expression Pattern and Its Interaction with NbPTP1 during Microsporidia Nosema bombycis Polar Tube Extrusion
© 2019 International Society of Protistologists..
Nosema bombycis (Nb) is a deadly species of microsporidia capable of causing pébrine, leading to heavy losses in sericulture. Germination is an important biological event in the invasion process of microsporidia. Septins, a family of membrane-associated proteins, play a critical role in tissue invasion and have been recognized as a virulence factor in numerous pathogens. Previous work in our laboratory has shown that Nosema bombycis septin2 (Nbseptin2) interacts with subtilisin-like protease 2 (NbSLP2). Herein, we found that Nbseptin2 was mainly associated with the plasma membrane in spores. Following spore germination, Nbseptin2 was found to co-localize with polar tube protein 1 (NbPTP1) at the polar cap and proximal zone of the polar tube. Co-immunoprecipitation and yeast two-hybrid analysis further confirmed that Nbseptin2 interacted with NbPTP1. The translocation and interaction of Nbseptin2 in the spores suggest that Nbseptin2 may play a significant role in microsporidia polar tube extrusion process. Our findings improve understanding of the mechanisms underlying microsporidia germination.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:67 |
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| Enthalten in: |
The Journal of eukaryotic microbiology - 67(2020), 1 vom: 22. Jan., Seite 45-53 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Fangyan [VerfasserIn] |
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| Links: |
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| Themen: |
Carrier Proteins |
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| Anmerkungen: |
Date Completed 11.09.2020 Date Revised 11.09.2020 published: Print-Electronic GENBANK: EOB13962 Citation Status MEDLINE |
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| doi: |
10.1111/jeu.12752 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM299452034 |
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| 245 | 1 | 0 | |a Nbseptin2 Expression Pattern and Its Interaction with NbPTP1 during Microsporidia Nosema bombycis Polar Tube Extrusion |
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| 520 | |a © 2019 International Society of Protistologists. | ||
| 520 | |a Nosema bombycis (Nb) is a deadly species of microsporidia capable of causing pébrine, leading to heavy losses in sericulture. Germination is an important biological event in the invasion process of microsporidia. Septins, a family of membrane-associated proteins, play a critical role in tissue invasion and have been recognized as a virulence factor in numerous pathogens. Previous work in our laboratory has shown that Nosema bombycis septin2 (Nbseptin2) interacts with subtilisin-like protease 2 (NbSLP2). Herein, we found that Nbseptin2 was mainly associated with the plasma membrane in spores. Following spore germination, Nbseptin2 was found to co-localize with polar tube protein 1 (NbPTP1) at the polar cap and proximal zone of the polar tube. Co-immunoprecipitation and yeast two-hybrid analysis further confirmed that Nbseptin2 interacted with NbPTP1. The translocation and interaction of Nbseptin2 in the spores suggest that Nbseptin2 may play a significant role in microsporidia polar tube extrusion process. Our findings improve understanding of the mechanisms underlying microsporidia germination | ||
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| 700 | 1 | |a Dang, Xiaoqun |e verfasserin |4 aut | |
| 700 | 1 | |a Meng, Xianzhi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Rong |e verfasserin |4 aut | |
| 700 | 1 | |a Bao, Jialing |e verfasserin |4 aut | |
| 700 | 1 | |a Long, Mengxian |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Tian |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Qiang |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Guoqing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Zeyang |e verfasserin |4 aut | |
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