LncRNA MIR155HG regulates M1/M2 macrophage polarization in chronic obstructive pulmonary disease
Copyright © 2019 The Authors. Published by Elsevier Masson SAS.. All rights reserved..
BACKGROUND: Macrophages play a crucial role in inflammatory diseases, including chronic obstructive pulmonary disease (COPD). MIR155 host gene (MIR155HG), a novel long non-coding RNA (lncRNA), has been recognized as a regulator of macrophage polarization, we thus investigated its role in COPD.
METHODS: We used granulocyte-macrophage colony-stimulating factor (GM-CSF) to induce peripheral blood mononuclear cells (PBMCs)-derived macrophages obtained from COPD patients and normal controls. Quantitative real-time PCR (QRT-PCR) was used to detect the expressions of MIR155HG and M1/M2 macrophage markers. The quantification of M1 and M2 macrophages was analyzed by flow cytometry. Enzyme-linked immunosorbent assay (ELISA) was conducted for testing the concentration of inflammatory cytokines.
RESULTS: MIR155HG was highly expressed in GM-CSF-induced macrophages of COPD patients. Further investigation demonstrated that MIR155HG overexpression promoted GM-CSF-induced M1 macrophage polarization and the release of pro-inflammatory cytokines. However, the knockdown of MIR-155HG could inhibit the polarization of M1 macrophages and increase M2 macrophage polarization.
CONCLUSION: LncRNA MIR155HG modulated GM-CSF-mediated M1/M2 macrophage polarization in COPD progression.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2019 |
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Erschienen: |
2019 |
Enthalten in: |
Zur Gesamtaufnahme - volume:117 |
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Enthalten in: |
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie - 117(2019) vom: 15. Sept., Seite 109015 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Li, Nannan [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 28.01.2020 Date Revised 28.01.2020 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.biopha.2019.109015 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM298240637 |
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520 | |a Copyright © 2019 The Authors. Published by Elsevier Masson SAS.. All rights reserved. | ||
520 | |a BACKGROUND: Macrophages play a crucial role in inflammatory diseases, including chronic obstructive pulmonary disease (COPD). MIR155 host gene (MIR155HG), a novel long non-coding RNA (lncRNA), has been recognized as a regulator of macrophage polarization, we thus investigated its role in COPD | ||
520 | |a METHODS: We used granulocyte-macrophage colony-stimulating factor (GM-CSF) to induce peripheral blood mononuclear cells (PBMCs)-derived macrophages obtained from COPD patients and normal controls. Quantitative real-time PCR (QRT-PCR) was used to detect the expressions of MIR155HG and M1/M2 macrophage markers. The quantification of M1 and M2 macrophages was analyzed by flow cytometry. Enzyme-linked immunosorbent assay (ELISA) was conducted for testing the concentration of inflammatory cytokines | ||
520 | |a RESULTS: MIR155HG was highly expressed in GM-CSF-induced macrophages of COPD patients. Further investigation demonstrated that MIR155HG overexpression promoted GM-CSF-induced M1 macrophage polarization and the release of pro-inflammatory cytokines. However, the knockdown of MIR-155HG could inhibit the polarization of M1 macrophages and increase M2 macrophage polarization | ||
520 | |a CONCLUSION: LncRNA MIR155HG modulated GM-CSF-mediated M1/M2 macrophage polarization in COPD progression | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Chronic obstructive pulmonary disease | |
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