Colonization of Barley Grain by Penicillium verrucosum and Ochratoxin A Formation in the Presence of Competing Fungi

Colonization of barley grain by Penicillium verrucosum and the formation of ochratoxin A were studied, both in pure culture and when paired with Aspergillus flavus , Fusarium sporotrichioides , and Hyphopichia burtonii , at 20° and 30°C and at 0.97, 0.95 and 0.90 aw over a 3-week period. Grain colonization was assessed on the basis of visible molding, seed infection, and numbers of CFU and by observing hyphal extension on the grain surface by scanning electron microscopy. Ochratoxin A concentrations were assayed by enzyme-linked immunosorbent assay using a monoclonal antibody. Germination of P. verrucosum spores was unaffected by the presence of other species. However, seed infection under most conditions was markedly decreased, relative to pure culture, by the presence of A. flavus and H. burtonii , but only slightly by F. sporotrichioides . The number of CFU of P. verrucosum was only slightly decreased in the presence of other species under most conditions. Generally, production of ochratoxin A by P. verrucosum was inhibited, sometimes significantly, in the presence of A. flavus and H. burtonii , but was changed only slightly by the presence of F. sporotrichioides . There was occasionally temporary enhancement in ochratoxin A production with all species during the 3-week incubation period.

Medienart:

E-Artikel

Erscheinungsjahr:

1996

Erschienen:

1996

Enthalten in:

Zur Gesamtaufnahme - volume:59

Enthalten in:

Journal of food protection - 59(1996), 12 vom: 15. Dez., Seite 1311-1317

Sprache:

Englisch

Beteiligte Personen:

Ramakrishna, Nannapaneni [VerfasserIn]
Lacey, John [VerfasserIn]
Smith, John E [VerfasserIn]

Links:

Volltext

Themen:

Barley grain
Fungal competition
Journal Article
Ochratoxin A

Anmerkungen:

Date Revised 08.03.2023

published: Print

Citation Status PubMed-not-MEDLINE

doi:

10.4315/0362-028X-59.12.1311

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM298121867