Long non-coding RNA MALAT1 sponges microRNA-429 to regulate apoptosis of hippocampal neurons in hypoxic-ischemic brain damage by regulating WNT1
Copyright © 2019. Published by Elsevier Inc..
Hypoxic-ischemic brain damage (HIBD) is a common neurological disorder. Emerging reports reveal that long non-coding RNAs and microRNAs (miRs) are implicated in the progress of HIBD. In this study we tried to ascertain whether lncRNA MALAT1, with the involvement of miR-429 and WNT1, affects HIBD. Initially, a HIBD mouse model was established. Then, we treated HIBD mice with dexmedetomidine (DEX) and then up- or down-regulated the expression of MALAT1, miR-429 and WNT1 in HIBD mice and neurons. Meanwhile, brain injury and hippocampal neuronal apoptosis were evaluated. Moreover, the interaction among MALAT1, miR-429 and WNT1 in HIBD was investigated. MALAT1 and WNT1 were high-expressed in brain tissues of HIBD mice while miR-429 was low-expressed in brain tissues from HIBD mice. Interestingly, MALAT1 silencing was observed to enhance the cerebral protection of DEX against HIBD. In addition, it was confirmed that MALAT1 sponged miR-429 downregulating expression of miR-429, thereby promoting apoptosis of hippocampal neurons. This effect was achieved through up-regulating the level of WNT1. Taken together, this study demonstrates that silencing of MALAT1 enhances the cerebral protection of DEX against HIBD by suppressing WNT1 expression through miR-429.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2019 |
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Erschienen: |
2019 |
Enthalten in: |
Zur Gesamtaufnahme - volume:152 |
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Enthalten in: |
Brain research bulletin - 152(2019) vom: 01. Okt., Seite 1-10 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Fang, Hua [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 14.08.2020 Date Revised 14.08.2020 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.brainresbull.2019.06.004 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM298022605 |
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520 | |a Hypoxic-ischemic brain damage (HIBD) is a common neurological disorder. Emerging reports reveal that long non-coding RNAs and microRNAs (miRs) are implicated in the progress of HIBD. In this study we tried to ascertain whether lncRNA MALAT1, with the involvement of miR-429 and WNT1, affects HIBD. Initially, a HIBD mouse model was established. Then, we treated HIBD mice with dexmedetomidine (DEX) and then up- or down-regulated the expression of MALAT1, miR-429 and WNT1 in HIBD mice and neurons. Meanwhile, brain injury and hippocampal neuronal apoptosis were evaluated. Moreover, the interaction among MALAT1, miR-429 and WNT1 in HIBD was investigated. MALAT1 and WNT1 were high-expressed in brain tissues of HIBD mice while miR-429 was low-expressed in brain tissues from HIBD mice. Interestingly, MALAT1 silencing was observed to enhance the cerebral protection of DEX against HIBD. In addition, it was confirmed that MALAT1 sponged miR-429 downregulating expression of miR-429, thereby promoting apoptosis of hippocampal neurons. This effect was achieved through up-regulating the level of WNT1. Taken together, this study demonstrates that silencing of MALAT1 enhances the cerebral protection of DEX against HIBD by suppressing WNT1 expression through miR-429 | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Apoptosis | |
650 | 4 | |a Dexmedetomidine | |
650 | 4 | |a Hippocampal neurons | |
650 | 4 | |a Hypoxic-ischemic brain damage | |
650 | 4 | |a Long non-coding RNA MALAT1 | |
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650 | 4 | |a WNT1 | |
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650 | 7 | |a Malat1 long non-coding RNA, mouse |2 NLM | |
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700 | 1 | |a He, Ming-Hai |e verfasserin |4 aut | |
700 | 1 | |a Yan, Jian-Yong |e verfasserin |4 aut | |
700 | 1 | |a Yang, Miao |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Fang-Xiang |e verfasserin |4 aut | |
700 | 1 | |a Wang, Ru-Rong |e verfasserin |4 aut | |
700 | 1 | |a Wang, Quan-Yun |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Jian-Ping |e verfasserin |4 aut | |
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