Long non-coding RNA MALAT1 sponges microRNA-429 to regulate apoptosis of hippocampal neurons in hypoxic-ischemic brain damage by regulating WNT1

Copyright © 2019. Published by Elsevier Inc..

Hypoxic-ischemic brain damage (HIBD) is a common neurological disorder. Emerging reports reveal that long non-coding RNAs and microRNAs (miRs) are implicated in the progress of HIBD. In this study we tried to ascertain whether lncRNA MALAT1, with the involvement of miR-429 and WNT1, affects HIBD. Initially, a HIBD mouse model was established. Then, we treated HIBD mice with dexmedetomidine (DEX) and then up- or down-regulated the expression of MALAT1, miR-429 and WNT1 in HIBD mice and neurons. Meanwhile, brain injury and hippocampal neuronal apoptosis were evaluated. Moreover, the interaction among MALAT1, miR-429 and WNT1 in HIBD was investigated. MALAT1 and WNT1 were high-expressed in brain tissues of HIBD mice while miR-429 was low-expressed in brain tissues from HIBD mice. Interestingly, MALAT1 silencing was observed to enhance the cerebral protection of DEX against HIBD. In addition, it was confirmed that MALAT1 sponged miR-429 downregulating expression of miR-429, thereby promoting apoptosis of hippocampal neurons. This effect was achieved through up-regulating the level of WNT1. Taken together, this study demonstrates that silencing of MALAT1 enhances the cerebral protection of DEX against HIBD by suppressing WNT1 expression through miR-429.

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:152

Enthalten in:

Brain research bulletin - 152(2019) vom: 01. Okt., Seite 1-10

Sprache:

Englisch

Beteiligte Personen:

Fang, Hua [VerfasserIn]
Li, Hua-Feng [VerfasserIn]
He, Ming-Hai [VerfasserIn]
Yan, Jian-Yong [VerfasserIn]
Yang, Miao [VerfasserIn]
Zhang, Fang-Xiang [VerfasserIn]
Wang, Ru-Rong [VerfasserIn]
Wang, Quan-Yun [VerfasserIn]
Zhang, Jian-Ping [VerfasserIn]

Links:

Volltext

Themen:

67VB76HONO
Apoptosis
Dexmedetomidine
Hippocampal neurons
Hypoxic-ischemic brain damage
Journal Article
Long non-coding RNA MALAT1
MIRN429 microRNA, mouse
Malat1 long non-coding RNA, mouse
MicroRNA-429
MicroRNAs
RNA, Long Noncoding
WNT1
Wnt1 Protein
Wnt1 protein, mouse

Anmerkungen:

Date Completed 14.08.2020

Date Revised 14.08.2020

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.brainresbull.2019.06.004

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM298022605