Residual Negative Pressure in Vacuum Blood-Collection Tube and Hemolysis in Pediatric Blood Specimens
© American Society for Clinical Pathology 2019. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
OBJECTIVE: To determine a method to reduce specimen hemolysis rates in pediatric blood specimens.
METHODS: A total of 290 blood specimens from pediatric patients were classified into the capped group or uncapped group. The hemolysis index and levels of lactate dehydrogenase (LDH) were measured using an automated biochemical analyzer. Also, we performed a paired test to measure the concentration of free hemoglobin in specimens from 25 randomly selected healthy adult volunteers, using a direct spectrophotometric technique.
RESULTS: The hemolytic rate of capped specimens was 2-fold higher than that of uncapped specimens. We found significant differences for LDH. Also, there was a significant difference in the concentration of free hemoglobin in the random-volunteers test.
CONCLUSIONS: Eliminating the residual negative pressure of vacuum blood-collection tubes was effective at reducing the macrohemolysis and/or microhemolysis rate.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:51 |
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| Enthalten in: |
Laboratory medicine - 51(2020), 1 vom: 02. Jan., Seite 41-46 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hu, Jing [VerfasserIn] |
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| Links: |
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| Themen: |
Blood collection tube |
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| Anmerkungen: |
Date Completed 03.06.2020 Date Revised 03.06.2020 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/labmed/lmz026 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM298020912 |
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| 500 | |a Date Revised 03.06.2020 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © American Society for Clinical Pathology 2019. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a OBJECTIVE: To determine a method to reduce specimen hemolysis rates in pediatric blood specimens | ||
| 520 | |a METHODS: A total of 290 blood specimens from pediatric patients were classified into the capped group or uncapped group. The hemolysis index and levels of lactate dehydrogenase (LDH) were measured using an automated biochemical analyzer. Also, we performed a paired test to measure the concentration of free hemoglobin in specimens from 25 randomly selected healthy adult volunteers, using a direct spectrophotometric technique | ||
| 520 | |a RESULTS: The hemolytic rate of capped specimens was 2-fold higher than that of uncapped specimens. We found significant differences for LDH. Also, there was a significant difference in the concentration of free hemoglobin in the random-volunteers test | ||
| 520 | |a CONCLUSIONS: Eliminating the residual negative pressure of vacuum blood-collection tubes was effective at reducing the macrohemolysis and/or microhemolysis rate | ||
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| 650 | 4 | |a residual negative pressure | |
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| 700 | 1 | |a Cai, Ying-Mu |e verfasserin |4 aut | |
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