Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5
Copyright © 2019. Published by Elsevier Inc..
Anti-biofilm peptides are a subset of antimicrobial peptides and represent promising broad-spectrum agents for the treatment of bacterial biofilms, though some display host toxicity in vivo. Here we evaluated nanogels composed of modified hyaluronic acid for the encapsulation of the anti-biofilm peptide DJK-5 in vivo. Nanogels of 174 to 194 nm encapsulating 33-60% of peptide were created. Efficacy and toxicity of the nanogels were tested in vivo employing a murine abscess model of a Pseudomonas aeruginosa LESB58 high bacterial density infection. The dose of DJK-5 that could be administered intravenously to mice without inducing toxicity was more than doubled after encapsulation in nanogels. Upon subcutaneous administration, the toxicity of the DJK-5 in nanogels was decreased four-fold compared to non-formulated peptide, without compromising the anti-abscess effect of DJK-5. These findings support the use of nanogels to increase the safety of antimicrobial and anti-biofilm peptides after intravenous and subcutaneous administration.
| Media Type: |
Electronic Article |
|---|
| Year of Publication: |
2019 |
|---|---|
| Publication: |
2019 |
| Contained In: |
To Main Record - volume:20 |
|---|---|
| Contained In: |
Nanomedicine : nanotechnology, biology, and medicine - 20(2019) vom: 15. Aug., Seite 102022 |
| Language: |
English |
|---|
| Contributors: |
Kłodzińska, Sylvia N [Author] |
|---|
| Links: |
|---|
| Notes: |
Date Completed 04.03.2020 Date Revised 04.03.2020 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.nano.2019.102022 |
|---|
| funding: |
|
|---|---|
| Supporting institution / Project title: |
|
| PPN (Catalogue-ID): |
NLM297877739 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM297877739 | ||
| 003 | DE-627 | ||
| 005 | 20231225092938.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.nano.2019.102022 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0992.xml |
| 035 | |a (DE-627)NLM297877739 | ||
| 035 | |a (NLM)31170510 | ||
| 035 | |a (PII)S1549-9634(19)30106-6 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kłodzińska, Sylvia N |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5 |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 04.03.2020 | ||
| 500 | |a Date Revised 04.03.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019. Published by Elsevier Inc. | ||
| 520 | |a Anti-biofilm peptides are a subset of antimicrobial peptides and represent promising broad-spectrum agents for the treatment of bacterial biofilms, though some display host toxicity in vivo. Here we evaluated nanogels composed of modified hyaluronic acid for the encapsulation of the anti-biofilm peptide DJK-5 in vivo. Nanogels of 174 to 194 nm encapsulating 33-60% of peptide were created. Efficacy and toxicity of the nanogels were tested in vivo employing a murine abscess model of a Pseudomonas aeruginosa LESB58 high bacterial density infection. The dose of DJK-5 that could be administered intravenously to mice without inducing toxicity was more than doubled after encapsulation in nanogels. Upon subcutaneous administration, the toxicity of the DJK-5 in nanogels was decreased four-fold compared to non-formulated peptide, without compromising the anti-abscess effect of DJK-5. These findings support the use of nanogels to increase the safety of antimicrobial and anti-biofilm peptides after intravenous and subcutaneous administration | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Biofilm | |
| 650 | 4 | |a Cationic peptide | |
| 650 | 4 | |a Drug delivery | |
| 650 | 4 | |a Nanogel | |
| 650 | 4 | |a Pseudomonas aeruginosa | |
| 650 | 7 | |a Biocompatible Materials |2 NLM | |
| 650 | 7 | |a DJK-5 peptide |2 NLM | |
| 650 | 7 | |a Nanogels |2 NLM | |
| 650 | 7 | |a Oligopeptides |2 NLM | |
| 650 | 7 | |a Reactive Nitrogen Species |2 NLM | |
| 650 | 7 | |a Reactive Oxygen Species |2 NLM | |
| 650 | 7 | |a Hyaluronic Acid |2 NLM | |
| 650 | 7 | |a 9004-61-9 |2 NLM | |
| 700 | 1 | |a Pletzer, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Rahanjam, Negin |e verfasserin |4 aut | |
| 700 | 1 | |a Rades, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Hancock, Robert E W |e verfasserin |4 aut | |
| 700 | 1 | |a Nielsen, Hanne M |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nanomedicine : nanotechnology, biology, and medicine |d 2005 |g 20(2019) vom: 15. Aug., Seite 102022 |w (DE-627)NLM16062021X |x 1549-9642 |7 nnns |
| 773 | 1 | 8 | |g volume:20 |g year:2019 |g day:15 |g month:08 |g pages:102022 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.nano.2019.102022 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 20 |j 2019 |b 15 |c 08 |h 102022 | ||