Dynamic control of enhancer activity drives stage-specific gene expression during flower morphogenesis

Enhancers are critical for developmental stage-specific gene expression, but their dynamic regulation in plants remains poorly understood. Here we compare genome-wide localization of H3K27ac, chromatin accessibility and transcriptomic changes during flower development in Arabidopsis. H3K27ac prevalently marks promoter-proximal regions, suggesting that H3K27ac is not a hallmark for enhancers in Arabidopsis. We provide computational and experimental evidence to confirm that distal DNase І hypersensitive sites are predictive of enhancers. The predicted enhancers are highly stage-specific across flower development, significantly associated with SNPs for flowering-related phenotypes, and conserved across crucifer species. Through the integration of genome-wide transcription factor (TF) binding datasets, we find that floral master regulators and stage-specific TFs are largely enriched at developmentally dynamic enhancers. Finally, we show that enhancer clusters and intronic enhancers significantly associate with stage-specific gene regulation by floral master TFs. Our study provides insights into the functional flexibility of enhancers during plant development, as well as hints to annotate plant enhancers.

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:10

Enthalten in:

Nature communications - 10(2019), 1 vom: 12. Apr., Seite 1705

Sprache:

Englisch

Beteiligte Personen:

Yan, Wenhao [VerfasserIn]
Chen, Dijun [VerfasserIn]
Schumacher, Julia [VerfasserIn]
Durantini, Diego [VerfasserIn]
Engelhorn, Julia [VerfasserIn]
Chen, Ming [VerfasserIn]
Carles, Cristel C [VerfasserIn]
Kaufmann, Kerstin [VerfasserIn]

Links:

Volltext

Themen:

Histones
Journal Article
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 09.05.2019

Date Revised 09.01.2021

published: Electronic

Citation Status MEDLINE

doi:

10.1038/s41467-019-09513-2

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM296008249