Lack of association of novel mutation Asp397Gly in aftB gene with ethambutol resistance in clinical isolates of Mycobacterium tuberculosis
Copyright © 2019 Elsevier Ltd. All rights reserved..
To discover additional genotypic indicators for ethambutol (EMB) resistant M. tuberculosis, we studied polymorphisms in arabinofuranosyl transferase encoding genes aftA (Rv3792), aftB (Rv3805) and aftC (Rv2673) in 38 EMB resistant and 34 EMB susceptible isolates from India and a repository established by the World Health Organization (WHO) Special Programme for Research and Training in Tropical Disease (TDR) by DNA sequencing. The results were correlated with the minimum inhibitory concentration (MIC) of EMB and mutations in embB (Rv3795). The most common non-synonymous polymorphism identified in aftB was Asp397Gly in 12/38 (31.6%) EMB resistant and 3/34 (8.8%) EMB susceptible isolates. Interestingly, 10/12 (83.3%) EMB resistant isolates with aftB Asp397Gly mutation also carried embB306, embB402 or embB497 mutations. Association of Asp397Gly polymorphism with EMB resistance was statistically significant (p 0.0216). However, overexpression of the mutant aftB in M. tuberculosis H37Rv did not exhibit any change in the MIC. Whole genome sequencing of a panel of Indian isolates and SNP cluster grouping (SCG) of TDR strains revealed an association between aftB mutation Asp397Gly and Beijing genotype or SCG2, a cluster group representing the Beijing genotype. To conclude, though aftBAsp397Gly mutation is not associated with EMB resistance, this mutation may be a phylogenetic marker for the Beijing clade.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2019 |
|---|---|
| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:115 |
|---|---|
| Enthalten in: |
Tuberculosis (Edinburgh, Scotland) - 115(2019) vom: 01. März, Seite 49-55 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Giri, Astha [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 07.01.2020 Date Revised 07.01.2020 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.tube.2019.01.004 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM295698799 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM295698799 | ||
| 003 | DE-627 | ||
| 005 | 20231225084226.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.tube.2019.01.004 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0985.xml |
| 035 | |a (DE-627)NLM295698799 | ||
| 035 | |a (NLM)30948176 | ||
| 035 | |a (PII)S1472-9792(18)30424-4 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Giri, Astha |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Lack of association of novel mutation Asp397Gly in aftB gene with ethambutol resistance in clinical isolates of Mycobacterium tuberculosis |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 07.01.2020 | ||
| 500 | |a Date Revised 07.01.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2019 Elsevier Ltd. All rights reserved. | ||
| 520 | |a To discover additional genotypic indicators for ethambutol (EMB) resistant M. tuberculosis, we studied polymorphisms in arabinofuranosyl transferase encoding genes aftA (Rv3792), aftB (Rv3805) and aftC (Rv2673) in 38 EMB resistant and 34 EMB susceptible isolates from India and a repository established by the World Health Organization (WHO) Special Programme for Research and Training in Tropical Disease (TDR) by DNA sequencing. The results were correlated with the minimum inhibitory concentration (MIC) of EMB and mutations in embB (Rv3795). The most common non-synonymous polymorphism identified in aftB was Asp397Gly in 12/38 (31.6%) EMB resistant and 3/34 (8.8%) EMB susceptible isolates. Interestingly, 10/12 (83.3%) EMB resistant isolates with aftB Asp397Gly mutation also carried embB306, embB402 or embB497 mutations. Association of Asp397Gly polymorphism with EMB resistance was statistically significant (p 0.0216). However, overexpression of the mutant aftB in M. tuberculosis H37Rv did not exhibit any change in the MIC. Whole genome sequencing of a panel of Indian isolates and SNP cluster grouping (SCG) of TDR strains revealed an association between aftB mutation Asp397Gly and Beijing genotype or SCG2, a cluster group representing the Beijing genotype. To conclude, though aftBAsp397Gly mutation is not associated with EMB resistance, this mutation may be a phylogenetic marker for the Beijing clade | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Beijing | |
| 650 | 4 | |a Ethambutol resistance | |
| 650 | 4 | |a Mycobacterium tuberculosis | |
| 650 | 4 | |a SNPs | |
| 650 | 4 | |a aftB | |
| 650 | 7 | |a Antitubercular Agents |2 NLM | |
| 650 | 7 | |a Ethambutol |2 NLM | |
| 650 | 7 | |a 8G167061QZ |2 NLM | |
| 650 | 7 | |a Pentosyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.4.2.- |2 NLM | |
| 650 | 7 | |a arabinosyltransferase |2 NLM | |
| 650 | 7 | |a EC 2.4.2.- |2 NLM | |
| 700 | 1 | |a Safi, Hassan |e verfasserin |4 aut | |
| 700 | 1 | |a Cabibbe, Andrea Maurizio |e verfasserin |4 aut | |
| 700 | 1 | |a Gupta, Shraddha |e verfasserin |4 aut | |
| 700 | 1 | |a Narang, Anshika |e verfasserin |4 aut | |
| 700 | 1 | |a Tyagi, Gaurav |e verfasserin |4 aut | |
| 700 | 1 | |a Shrivastava, Kamal |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar, Chanchal |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar Sharma, Naresh |e verfasserin |4 aut | |
| 700 | 1 | |a Lingaraju, Subramanya |e verfasserin |4 aut | |
| 700 | 1 | |a Trovato, Alberto |e verfasserin |4 aut | |
| 700 | 1 | |a Battaglia, Simone |e verfasserin |4 aut | |
| 700 | 1 | |a Cirillo, Daniela Maria |e verfasserin |4 aut | |
| 700 | 1 | |a Bose, Mridula |e verfasserin |4 aut | |
| 700 | 1 | |a Alland, David |e verfasserin |4 aut | |
| 700 | 1 | |a Varma-Basil, Mandira |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Tuberculosis (Edinburgh, Scotland) |d 2001 |g 115(2019) vom: 01. März, Seite 49-55 |w (DE-627)NLM113651538 |x 1873-281X |7 nnns |
| 773 | 1 | 8 | |g volume:115 |g year:2019 |g day:01 |g month:03 |g pages:49-55 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.tube.2019.01.004 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 115 |j 2019 |b 01 |c 03 |h 49-55 | ||