Expression profile of several genes on ecdysteroidogenic pathway related to diapause in pupal stage of Bombyx mori bivoltine strain

Copyright © 2019 Elsevier B.V. All rights reserved..

Ecdysone is involved in regulation of embryonic diapause in the silkworm, Bombyx mori. However, its mechanism still remains unclear. To explore the role of ecdysteroidogenic pathway (EP) genes in diapause process of bivoltine B. mori, the eggs of "Qiufeng", a bivoltine strain, were used as the study materials and arranged into diapause eggs producers (DEPs) and non-diapause eggs producers (NDEPs), respectively. The differential expression of EP genes between two groups was analysed during the early pupal stage. The expression of Shadow was significantly increased in the NDEPs in day-3 pupae and reached the peak simultaneously, indicating that Shadow was in coincidence with diapause process. To validate this hypothesis, a repression of Shadow by RNA interference was performed in day-2 pupae of NDEPs. The expression of Shadow was downregulated by RNAi, and βFtz-F1, a downstream gene of EP, was also decreased. Furthermore, the genes encoding the kynurenine-synthetase were upregulated in the ovary, and Brown, AdenoK which link Shadow to the kynurenine-synthase gene were also upregulated in the fat body. The progeny eggs appeared a light purple colour at 48 h after oviposition, revealing a certain tendency to diapause. We speculate that inhibition of Shadow upregulates 3-hydroxy-kynurenine synthesis by increasing the expression of Brown and AdenoK. In addition, Shadow was cloned, and expressed in E. coli for further functional study of Shadow protein. Our study provided insight into the role of EP genes in the process of diapause of B. mori.

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:707

Enthalten in:

Gene - 707(2019) vom: 30. Juli, Seite 109-116

Sprache:

Englisch

Beteiligte Personen:

Zhu, Juan [VerfasserIn]
Chen, Yanrong [VerfasserIn]
Zhao, Qiaoling [VerfasserIn]
Tang, Shunming [VerfasserIn]
Huang, Jinshan [VerfasserIn]
Shen, Xingjia [VerfasserIn]

Links:

Volltext

Themen:

3-Hydroxy-kynurenine
9035-51-2
ATP-Binding Cassette Transporters
Bombyx mori
Comparative Study
Cytochrome P-450 Enzyme System
Development
Diapause
Ecdysteroid
Ecdysteroids
Insect Proteins
Journal Article

Anmerkungen:

Date Completed 24.06.2019

Date Revised 30.07.2019

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.gene.2019.03.054

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM295652268