Molecular Detection of Glycophorins A and B Variant Phenotypes and their Clinical Relevance

Copyright © 2019 Elsevier Inc. All rights reserved..

Crossover or conversion between the homologous regions of glycophorin A (GYPA) and glycophorin B (GYPB) gives rise to several different hybrid glycophorin genes encoding a number of different glycophorin variant phenotypes which bear low prevalence antigens in the MNS blood group system. GP.Mur is the main glycophorin variant phenotype which causes hemolytic transfusion reaction (HTR) and hemolytic disease of the fetus and newborn (HDFN) in East and Southeast Asians. The detection of glycophorin variant phenotypes using serological methods is limited to phenotyping reagents that are not commercially available. Moreover, the red blood cells used for antibody identification are usually of the GP.Mur phenotype. The current Polymerase Chain Reaction (PCR)-based methods and loop-mediated isothermal amplification (LAMP) are available alternatives to phenotyping that allow for the specific detection of glycophorin variant phenotypes. This review highlights the molecular detection method for glycophorins A and B variant phenotypes and their clinical relevance.

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:33

Enthalten in:

Transfusion medicine reviews - 33(2019), 2 vom: 01. Apr., Seite 118-124

Sprache:

Englisch

Beteiligte Personen:

Hassan, Siti Nazihahasma [VerfasserIn]
Thirumulu Ponnuraj, Kannan [VerfasserIn]
Mohamad, Suharni [VerfasserIn]
Hassan, Rosline [VerfasserIn]
Wan Ab Rahman, Wan Suriana [VerfasserIn]

Links:

Volltext

Themen:

GYPA protein, human
GYPB protein, human
Glycophorin variant phenotypes
Glycophorins
Hemolytic disease of the fetus and newborn
Hemolytic transfusion reaction
Hybrid glycophorins
Journal Article
MNS blood group system
MNSs Blood-Group System
Molecular method
Research Support, Non-U.S. Gov't
Review

Anmerkungen:

Date Completed 14.02.2020

Date Revised 14.02.2020

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.tmrv.2019.02.003

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM29532693X