Dyella dinghuensis sp. nov. and Dyella choica sp. nov., isolated from forest soil

Two aerobic, Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated as DHOA06T and 4 M-K27T, were isolated from soil samples collected from the forest of Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31' E 23° 10' N). Strains DHOA06T and 4 M-K27T grew at pH 4.5-7.0 (optimum, pH 5.0-6.0) and pH 4.5-6.5 (pH 6.0), respectively. Both strains grew at 12-37 °C (optimum, 28 °C) and NaCl levels up to 1.0 % (optimum 0 %, w/v). Phylogenetic analysis based on both 16S rRNA gene sequences and the concatenated partial atpD, gyrB andlepA gene sequences showed that strains DHOA06T and 4 M-K27T formed two isolated clades with members of the genus Dyella, but they each occupied a distinctive position within the genus. Strains DHOA06T and 4 M-K27T showed the highest 16S rRNA gene sequence similarities to Dyellacaseinilytica DHOB09T (98.7 %) and Dyellaacidisoli 4M-Z03T (98.8 %), respectively. DNA-DNA hybridization values of strains DHOA06T/DHOB09T and 4 M-K27T/4M-Z03T were 27.4±2.4 % and 38.8±1.0 %, respectively. Ubiquinone-8 was the only respiratory quinone detected in both strains. Their major fatty acids consisted of iso-C15 : 0, iso-C16 : 0 and iso-C17 : 1ω9c, and strain DHOA06T had iso-C17 : 0 in addition. Their polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminophospholipid, one unidentified phospholipid and two unidentified aminolipids, and strain DHOA06T had phosphatidylmethylethanolamine and one unidentified lipid in addition. The DNA G+C contents of strains DHOA06T and 4 M-K27T were 59.1 and 61.7 mol%, respectively. Based on the above results, we propose that strains DHOA06T and 4 M-K27T represent two novel species of the genus Dyella, namely Dyelladinghuensis sp. nov. (type strain DHOA06T = KCTC 52129T=NBRC 111978T) and Dyellachoica sp. nov. (type strain 4 M-K27T=GDMCC 1.1189T=LMG 30267T).

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:69

Enthalten in:

International journal of systematic and evolutionary microbiology - 69(2019), 5 vom: 10. Mai, Seite 1496-1503

Sprache:

Englisch

Beteiligte Personen:

Ou, Fang-Hong [VerfasserIn]
Gao, Zeng-Hong [VerfasserIn]
Chen, Mei-Hong [VerfasserIn]
Bi, Jie-Yi [VerfasserIn]
Qiu, Li-Hong [VerfasserIn]

Links:

Volltext

Themen:

1339-63-5
CQA993F7P8
Choica
DNA, Bacterial
Dinghuensis
Dyella
Fatty Acids
Journal Article
Phospholipids
Phylogeny
RNA, Ribosomal, 16S
Taxonomy
Ubiquinone
Ubiquinone 8

Anmerkungen:

Date Completed 23.05.2019

Date Revised 23.05.2019

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1099/ijsem.0.003356

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM295235713