Activation of the ERK/Creb/Bcl‑2 pathway protects periodontal ligament stem cells against hydrogen peroxide‑induced oxidative stress

Periodontal ligament stem cells (PDLSCs) are promising stem cells sources for regenerative medicine, particularly clinical periodontal ligament repair. It is critical to maintain high quality and a large quantity of PDLSCs for clinical usage. However, how PDLSCs respond to environmental stimuli, including reactive oxygen species (ROS), is poorly understood. The aim of the present study was to investigate how PDLSCs react to oxidative stress and the underlying mechanisms. Hydrogen peroxide‑induced oxidative stress was used to mimic a ROS increase in rat PDLSCs. The expression levels of Creb were detected under oxidative stress to examine the role that Creb serves in PDLSCs under oxidative stress. The present results demonstrated that the expression of Creb was reduced in a dose‑dependent manner in response to the H2O2 stimulus. Overexpressing Creb significantly reduced the ROS levels and protein expression levels of apoptotic genes in PDLSCs. The phosphorylation of the ERK pathway is indispensable in the activation of Creb‑induced protection. Our results revealed a protective role of Creb in ROS‑induced apoptosis, and validated the ERK/Creb/apoptosis regulator Bcl‑2 pathway works as an anti‑apoptotic signaling in PDLSCs. These findings will facilitate the in vitro culturing of PDLSCs for clinical usage and promote stem cell based therapy for periodontal tissue regeneration.

Medienart:

E-Artikel

Erscheinungsjahr:

2019

Erschienen:

2019

Enthalten in:

Zur Gesamtaufnahme - volume:19

Enthalten in:

Molecular medicine reports - 19(2019), 5 vom: 01. Mai, Seite 3649-3657

Sprache:

Englisch

Beteiligte Personen:

Fu, Xiaohui [VerfasserIn]
Feng, Yimiao [VerfasserIn]
Shao, Bingyi [VerfasserIn]
Zhang, Yanzhen [VerfasserIn]

Links:

Volltext

Themen:

BBX060AN9V
Caspases
Cyclic AMP Response Element-Binding Protein
EC 2.7.11.24
EC 3.4.22.-
Extracellular Signal-Regulated MAP Kinases
Hydrogen Peroxide
Journal Article
Proto-Oncogene Proteins c-bcl-2
Reactive Oxygen Species

Anmerkungen:

Date Completed 01.08.2019

Date Revised 25.02.2020

published: Print-Electronic

Citation Status MEDLINE

doi:

10.3892/mmr.2019.10027

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM295195282