Structural and evolutionary approaches to the design and optimization of fluorescence-based small molecule biosensors
Copyright © 2019 Elsevier Ltd. All rights reserved..
Biosensors that selectively report on the presence of specific small molecule analytes have applications in many fields of research, medicine and biotechnology. Here, we review recent advances and emerging approaches in the design and optimisation of genetically encoded fluorescence-based small molecule biosensors. We discuss how natural sensory proteins can be exploited to produce novel biosensors and the strategies for optimizing ligand specificity and fluorescence readout. Finally, we provide insight into high-throughput sensor optimisation and discuss the challenges that are faced when designing novel biosensors.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:57 |
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| Enthalten in: |
Current opinion in structural biology - 57(2019) vom: 29. Aug., Seite 31-38 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kaczmarski, Joe A [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Completed 29.06.2020 Date Revised 29.06.2020 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.sbi.2019.01.013 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM294501371 |
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| 520 | |a Biosensors that selectively report on the presence of specific small molecule analytes have applications in many fields of research, medicine and biotechnology. Here, we review recent advances and emerging approaches in the design and optimisation of genetically encoded fluorescence-based small molecule biosensors. We discuss how natural sensory proteins can be exploited to produce novel biosensors and the strategies for optimizing ligand specificity and fluorescence readout. Finally, we provide insight into high-throughput sensor optimisation and discuss the challenges that are faced when designing novel biosensors | ||
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| 700 | 1 | |a Jackson, Colin J |e verfasserin |4 aut | |
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