Possible Association between Cathepsin V and the Development of Placenta Accreta Spectrum Disorders
© 2019 S. Karger AG, Basel..
BACKGROUND/AIMS: The study aimed to evaluate molecular changes related to trophoblast adhesion in placenta accreta spectrum (PAS) disorders.
METHODS: A retrospective analysis of 10 PAS cases in which both the trophoblast adherent site and the non-adherent site were identified was performed in April 2010 and March 2013. Microarray analysis and reverse transcription polymerase chain reaction (RT-PCR) analyses were performed to extract upregulated genes in the adherent site. Gene expression changes were examined by immunohistochemistry.
RESULTS: Microarray analysis showed that 157 transcripts were > 3-fold upregulated, including the following: a disintegrin and metalloproteinase-28 (ADAM28), 3.10-fold; cathepsin V (CTSV), 3.73-fold; cathepsin S (CTSS), 3.46-fold; and matrix metalloproteinase-19 (MMP19), 3.41-fold. RT-PCR showed relatively high mRNA expressions. On immunohistochemistry, extravillous trophoblast (EVT) at the non-adherent site showed weak or no CTSV expression, whereas EVT that invaded myometrium at the adherent site showed strong expression (histological score, median [min-max], 115.6 [37.6-153.6] vs. 184.8 [56.4-222.8], p < 0.05). MMP19 showed moderate staining, with no difference between the adherent and non-adherent sites. ADAM28 and CTSS showed weak or no staining.
DISCUSSION: This limited study suggests that CTSV may be involved in the pathogenesis of PAS.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2019 |
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| Erschienen: |
2019 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:84 |
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| Enthalten in: |
Gynecologic and obstetric investigation - 84(2019), 4 vom: 31., Seite 396-406 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Matsukawa, Satoshi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 23.10.2019 Date Revised 23.10.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1159/000496609 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM293850615 |
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| 100 | 1 | |a Matsukawa, Satoshi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Possible Association between Cathepsin V and the Development of Placenta Accreta Spectrum Disorders |
| 264 | 1 | |c 2019 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 23.10.2019 | ||
| 500 | |a Date Revised 23.10.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2019 S. Karger AG, Basel. | ||
| 520 | |a BACKGROUND/AIMS: The study aimed to evaluate molecular changes related to trophoblast adhesion in placenta accreta spectrum (PAS) disorders | ||
| 520 | |a METHODS: A retrospective analysis of 10 PAS cases in which both the trophoblast adherent site and the non-adherent site were identified was performed in April 2010 and March 2013. Microarray analysis and reverse transcription polymerase chain reaction (RT-PCR) analyses were performed to extract upregulated genes in the adherent site. Gene expression changes were examined by immunohistochemistry | ||
| 520 | |a RESULTS: Microarray analysis showed that 157 transcripts were > 3-fold upregulated, including the following: a disintegrin and metalloproteinase-28 (ADAM28), 3.10-fold; cathepsin V (CTSV), 3.73-fold; cathepsin S (CTSS), 3.46-fold; and matrix metalloproteinase-19 (MMP19), 3.41-fold. RT-PCR showed relatively high mRNA expressions. On immunohistochemistry, extravillous trophoblast (EVT) at the non-adherent site showed weak or no CTSV expression, whereas EVT that invaded myometrium at the adherent site showed strong expression (histological score, median [min-max], 115.6 [37.6-153.6] vs. 184.8 [56.4-222.8], p < 0.05). MMP19 showed moderate staining, with no difference between the adherent and non-adherent sites. ADAM28 and CTSS showed weak or no staining | ||
| 520 | |a DISCUSSION: This limited study suggests that CTSV may be involved in the pathogenesis of PAS | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Observational Study | |
| 650 | 4 | |a Abnormally invasive placenta | |
| 650 | 4 | |a Adherent placenta | |
| 650 | 4 | |a Biomarker | |
| 650 | 4 | |a Pathogenesis | |
| 650 | 4 | |a Placenta creta | |
| 650 | 4 | |a Proteinase | |
| 650 | 7 | |a Cathepsins |2 NLM | |
| 650 | 7 | |a EC 3.4.- |2 NLM | |
| 650 | 7 | |a Cysteine Endopeptidases |2 NLM | |
| 650 | 7 | |a EC 3.4.22.- |2 NLM | |
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| 650 | 7 | |a EC 3.4.22.43 |2 NLM | |
| 650 | 7 | |a ADAM Proteins |2 NLM | |
| 650 | 7 | |a EC 3.4.24.- |2 NLM | |
| 650 | 7 | |a ADAM28 protein, human |2 NLM | |
| 650 | 7 | |a EC 3.4.24.- |2 NLM | |
| 650 | 7 | |a Matrix Metalloproteinases, Secreted |2 NLM | |
| 650 | 7 | |a EC 3.4.24.- |2 NLM | |
| 650 | 7 | |a matrix metalloproteinase 19 |2 NLM | |
| 650 | 7 | |a EC 3.4.24.- |2 NLM | |
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| 700 | 1 | |a Kotani, Tomomi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jingwen |e verfasserin |4 aut | |
| 700 | 1 | |a Miki, Rika |e verfasserin |4 aut | |
| 700 | 1 | |a Moriyama, Yoshinori |e verfasserin |4 aut | |
| 700 | 1 | |a Nakano, Tomoko |e verfasserin |4 aut | |
| 700 | 1 | |a Mano, Yukio |e verfasserin |4 aut | |
| 700 | 1 | |a Tsuda, Hiroyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Tamakoshi, Koji |e verfasserin |4 aut | |
| 700 | 1 | |a Kikkawa, Fumitaka |e verfasserin |4 aut | |
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