Asparagine synthetase expression is associated with the sensitivity to asparaginase in extranodal natural killer/T-cell lymphoma in vivo and in vitro

BACKGROUND: Although asparagine synthetase (AsnS) is associated with drug resistance in leukemia, its function in extranodal natural killer (NK)/T-cell lymphoma (ENKTL) remains unclear.

METHODS: The present study investigated the relationship between baseline AsnS mRNA levels and response to asparaginase in ENKTL cell lines. It also determined whether upregulating or downregulating the AsnS mRNA level induces or reverses asparaginase-resistant phenotype.

RESULTS: Interestingly, considerable differences were observed in the sensitivity to asparaginase of the five ENKTL cell lines. The AsnS expression levels were positively correlated with the IC50 values. In addition, the asparaginase resistance was induced or reversed by upregulating or downregulating the AsnS mRNA level in vivo and in vitro. Functional analyses indicated that AsnS did not affect the proliferation and apoptosis of ENKTL cells in the absence of asparaginase.

CONCLUSION: Together, the data stress the importance of AsnS in the sensitivity to asparaginase in ENKTL and suggest a different therapeutic strategy for patients with a different level of AsnS expression.

Medienart:

E-Artikel

Erscheinungsjahr:

2018

Erschienen:

2018

Enthalten in:

Zur Gesamtaufnahme - volume:11

Enthalten in:

OncoTargets and therapy - 11(2018) vom: 20., Seite 6605-6615

Sprache:

Englisch

Beteiligte Personen:

Liu, Wen-Jian [VerfasserIn]
Wang, Hua [VerfasserIn]
Peng, Xiong-Wen [VerfasserIn]
Wang, Wei-da [VerfasserIn]
Liu, Na-Wei [VerfasserIn]
Wang, Yang [VerfasserIn]
Lu, Yue [VerfasserIn]

Links:

Volltext

Themen:

Asparaginase resistance
Asparagine synthetase
Extranodal natural killer/T-cell lymphomax
Journal Article

Anmerkungen:

Date Revised 21.03.2022

published: Electronic-eCollection

Citation Status PubMed-not-MEDLINE

doi:

10.2147/OTT.S155930

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM289836697