Functional redundancy in Echinocandin B in-cluster transcription factor ecdB of Emericella rugulosa NRRL 11440
Echinocandin B is a potent antifungal against the majority of fungal pathogens and its biosynthesis occurred by ecd and hty gene clusters in Emericella rugulosa NRRL 11440. We elucidated the functional necessity of in-clustered transcription factor; ecdB in the production of echinocandin B. We deleted the ecdB gene and found that ΔecdB mutant has no significant effect on echinocandin B production. The expression level of most of the ecd and hty cluster genes was not significantly altered except few of them up-regulated in knockout strain. The complete abrogation in ecdB gene expression was observed in ΔecdB strain. However, the interactions of purified EcdB protein with DNA sequence of ecdA, ecdH, ecdK and ecdI promoter was confirmed in-vitro. Our results conclude that EcdB protein in-vitro binds to the ecdA, ecdH, ecdK and ecdI promoter but in-vivo, it could not significantly affect the gene expression and echinocandin B production in Emericella rugulosa.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2018 |
|---|---|
| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
|---|---|
| Enthalten in: |
Biotechnology reports (Amsterdam, Netherlands) - 19(2018) vom: 09. Sept., Seite e00264 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Kumar, Arvind [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Revised 29.03.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1016/j.btre.2018.e00264 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM286346583 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM286346583 | ||
| 003 | DE-627 | ||
| 005 | 20240329232020.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.btre.2018.e00264 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1354.xml |
| 035 | |a (DE-627)NLM286346583 | ||
| 035 | |a (NLM)29992098 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kumar, Arvind |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Functional redundancy in Echinocandin B in-cluster transcription factor ecdB of Emericella rugulosa NRRL 11440 |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 29.03.2024 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Echinocandin B is a potent antifungal against the majority of fungal pathogens and its biosynthesis occurred by ecd and hty gene clusters in Emericella rugulosa NRRL 11440. We elucidated the functional necessity of in-clustered transcription factor; ecdB in the production of echinocandin B. We deleted the ecdB gene and found that ΔecdB mutant has no significant effect on echinocandin B production. The expression level of most of the ecd and hty cluster genes was not significantly altered except few of them up-regulated in knockout strain. The complete abrogation in ecdB gene expression was observed in ΔecdB strain. However, the interactions of purified EcdB protein with DNA sequence of ecdA, ecdH, ecdK and ecdI promoter was confirmed in-vitro. Our results conclude that EcdB protein in-vitro binds to the ecdA, ecdH, ecdK and ecdI promoter but in-vivo, it could not significantly affect the gene expression and echinocandin B production in Emericella rugulosa | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Arg, Arginine | |
| 650 | 4 | |a Biosynthetic regulation | |
| 650 | 4 | |a CTAB, cetyl trimethylammonium bromide | |
| 650 | 4 | |a ECB, echinocandin B | |
| 650 | 4 | |a Echinocandin B | |
| 650 | 4 | |a Emericella rugulosa | |
| 650 | 4 | |a Functional redundancy | |
| 650 | 4 | |a GMM, glucose minimal medium | |
| 650 | 4 | |a HPLC, high performance liquid chromatography | |
| 650 | 4 | |a ICU, intensive care unit | |
| 650 | 4 | |a In-clustered transcription factor | |
| 650 | 4 | |a MEGA, molecular evolutionary genetics analysis | |
| 650 | 4 | |a MP, maximum parsimony | |
| 650 | 4 | |a ORF, open reading frame | |
| 650 | 4 | |a PCR, polymerase chain reaction | |
| 650 | 4 | |a RT-PCR, reverse transcription polymerase chain reaction | |
| 650 | 4 | |a SPR, subtree-pruning-regrafting | |
| 650 | 4 | |a TF, transcription factor | |
| 650 | 4 | |a UTR, un-translated region | |
| 650 | 4 | |a WT, wild type | |
| 650 | 4 | |a YG, yeast glucose medium | |
| 650 | 4 | |a bp, base pair | |
| 650 | 4 | |a d, day | |
| 650 | 4 | |a h, hour | |
| 650 | 4 | |a kb, kilo base pair | |
| 700 | 1 | |a Jaiswal, Varun |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar, Vinay |e verfasserin |4 aut | |
| 700 | 1 | |a Dey, Amitava |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar, Antresh |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biotechnology reports (Amsterdam, Netherlands) |d 2014 |g 19(2018) vom: 09. Sept., Seite e00264 |w (DE-627)NLM241422434 |x 2215-017X |7 nnns |
| 773 | 1 | 8 | |g volume:19 |g year:2018 |g day:09 |g month:09 |g pages:e00264 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.btre.2018.e00264 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 19 |j 2018 |b 09 |c 09 |h e00264 | ||