De Novo Generation of Somatic Stem Cells by YAP/TAZ

Here we present protocols to isolate primary differentiated cells and turn them into stem/progenitor cells (SCs) of the same lineage by transient expression of the transcription factor YAP. With this method, luminal differentiated (LD) cells of the mouse mammary gland are converted into cells that exhibit molecular and functional properties of mammary SCs. YAP also turns fully differentiated pancreatic exocrine cells into pancreatic duct-like progenitors. Similarly, to endogenous, natural SCs, YAP-induced stem-like cells ("ySCs") can be eventually expanded as organoid cultures long term in vitro, without further need of ectopic YAP/TAZ, as ySCs are endowed with a heritable self-renewing SC-like state. The reprogramming procedure presented here offers the possibility to generate and expand in vitro progenitor cells of various tissue sources starting from differentiated cells. The straightforward expansion of somatic cells ex vivo has implications for regenerative medicine, for understanding mechanisms of tumor initiation and, more in general, for cell and developmental biology studies.

Medienart:

E-Artikel

Erscheinungsjahr:

2018

Erschienen:

2018

Enthalten in:

Zur Gesamtaufnahme - year:2018

Enthalten in:

Journal of visualized experiments : JoVE - (2018), 135 vom: 07. Mai

Sprache:

Englisch

Beteiligte Personen:

Panciera, Tito [VerfasserIn]
Azzolin, Luca [VerfasserIn]
Di Biagio, Daniele [VerfasserIn]
Totaro, Antonio [VerfasserIn]
Cordenonsi, Michelangelo [VerfasserIn]
Piccolo, Stefano [VerfasserIn]

Links:

Volltext

Themen:

Acyltransferases
Adaptor Proteins, Signal Transducing
Cell Cycle Proteins
EC 2.3.-
Journal Article
Phosphoproteins
Research Support, Non-U.S. Gov't
Tafazzin protein, mouse
Transcription Factors
Video-Audio Media
YAP-Signaling Proteins
Yap1 protein, mouse

Anmerkungen:

Date Completed 30.07.2018

Date Revised 29.01.2022

published: Electronic

Citation Status MEDLINE

doi:

10.3791/57462

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM284281204