Expression, purification and characterization of Rv3194c protein from Mycobacterium tuberculosis

Objective: PDZ[Post-synaptic density-95 (PSD-95), Drosophilia tumor suppressor protein diskslarge-1 (DLG), the tight junction protein zonula occludentes 1 (ZO-1)] signal protein was encoded by Rv3194c gene from Mycobacterium tuberculosis, and its ability to adhere M. tuberculosis was studied.

Methods: Rv3194c protein was expressed in prokaryotic system. Rv3194c protein was separately incubated with hyaluronic acid, chondroitin sulfate and collagen Ι overnight at different temperature (37, 38, 39, 40℃). Then component changes of culture supernatant were tested by Western blot and ELISA.

Results: Western blot showed that Rv3194c protein expressed in prokaryotic system, with a molecular weight of about 35 kDa, was mainly in soluble form. Western blot showed that His-Rv3194c protein in supernatant of 39℃ experimental group was significantly less than that of other experimental groups (37, 38, 40℃)(***P<0.001). ELISA showed that hyaluronic acid, chondroitin sulfate and collagen Ι in supernatant of 39℃ experimental group was significantly less than that of other experimental groups (37, 38, 40℃)(***P<0.001).

Conclusion: For the first time it was affirmed that Rv3194c protein with detected activity of adhesions in this study will be targeted to the development of the new anti-M. tuberculosis drug.

Medienart:

Artikel

Erscheinungsjahr:

2016

Erschienen:

2016

Enthalten in:

Zur Gesamtaufnahme - volume:56

Enthalten in:

Wei sheng wu xue bao = Acta microbiologica Sinica - 56(2016), 12 vom: 04. Dez., Seite 1847-55

Sprache:

Chinesisch

Beteiligte Personen:

Zhao, Dongyue [VerfasserIn]
Lin, Lili [VerfasserIn]
Wen, Fuli [VerfasserIn]

Themen:

9004-61-9
9007-28-7
9007-34-5
Bacterial Proteins
Chondroitin Sulfates
Collagen
Hyaluronic Acid
Journal Article

Anmerkungen:

Date Completed 24.05.2018

Date Revised 24.05.2018

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM283887672