Expression, purification and characterization of Rv3194c protein from Mycobacterium tuberculosis
Objective: PDZ[Post-synaptic density-95 (PSD-95), Drosophilia tumor suppressor protein diskslarge-1 (DLG), the tight junction protein zonula occludentes 1 (ZO-1)] signal protein was encoded by Rv3194c gene from Mycobacterium tuberculosis, and its ability to adhere M. tuberculosis was studied.
Methods: Rv3194c protein was expressed in prokaryotic system. Rv3194c protein was separately incubated with hyaluronic acid, chondroitin sulfate and collagen Ι overnight at different temperature (37, 38, 39, 40℃). Then component changes of culture supernatant were tested by Western blot and ELISA.
Results: Western blot showed that Rv3194c protein expressed in prokaryotic system, with a molecular weight of about 35 kDa, was mainly in soluble form. Western blot showed that His-Rv3194c protein in supernatant of 39℃ experimental group was significantly less than that of other experimental groups (37, 38, 40℃)(***P<0.001). ELISA showed that hyaluronic acid, chondroitin sulfate and collagen Ι in supernatant of 39℃ experimental group was significantly less than that of other experimental groups (37, 38, 40℃)(***P<0.001).
Conclusion: For the first time it was affirmed that Rv3194c protein with detected activity of adhesions in this study will be targeted to the development of the new anti-M. tuberculosis drug.
Medienart: |
Artikel |
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Erscheinungsjahr: |
2016 |
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Erschienen: |
2016 |
Enthalten in: |
Zur Gesamtaufnahme - volume:56 |
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Enthalten in: |
Wei sheng wu xue bao = Acta microbiologica Sinica - 56(2016), 12 vom: 04. Dez., Seite 1847-55 |
Sprache: |
Chinesisch |
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Beteiligte Personen: |
Zhao, Dongyue [VerfasserIn] |
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Themen: |
9004-61-9 |
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Anmerkungen: |
Date Completed 24.05.2018 Date Revised 24.05.2018 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM283887672 |
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100 | 1 | |a Zhao, Dongyue |e verfasserin |4 aut | |
245 | 1 | 0 | |a Expression, purification and characterization of Rv3194c protein from Mycobacterium tuberculosis |
264 | 1 | |c 2016 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 24.05.2018 | ||
500 | |a Date Revised 24.05.2018 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Objective: PDZ[Post-synaptic density-95 (PSD-95), Drosophilia tumor suppressor protein diskslarge-1 (DLG), the tight junction protein zonula occludentes 1 (ZO-1)] signal protein was encoded by Rv3194c gene from Mycobacterium tuberculosis, and its ability to adhere M. tuberculosis was studied | ||
520 | |a Methods: Rv3194c protein was expressed in prokaryotic system. Rv3194c protein was separately incubated with hyaluronic acid, chondroitin sulfate and collagen Ι overnight at different temperature (37, 38, 39, 40℃). Then component changes of culture supernatant were tested by Western blot and ELISA | ||
520 | |a Results: Western blot showed that Rv3194c protein expressed in prokaryotic system, with a molecular weight of about 35 kDa, was mainly in soluble form. Western blot showed that His-Rv3194c protein in supernatant of 39℃ experimental group was significantly less than that of other experimental groups (37, 38, 40℃)(***P<0.001). ELISA showed that hyaluronic acid, chondroitin sulfate and collagen Ι in supernatant of 39℃ experimental group was significantly less than that of other experimental groups (37, 38, 40℃)(***P<0.001) | ||
520 | |a Conclusion: For the first time it was affirmed that Rv3194c protein with detected activity of adhesions in this study will be targeted to the development of the new anti-M. tuberculosis drug | ||
650 | 4 | |a Journal Article | |
650 | 7 | |a Bacterial Proteins |2 NLM | |
650 | 7 | |a Hyaluronic Acid |2 NLM | |
650 | 7 | |a 9004-61-9 |2 NLM | |
650 | 7 | |a Chondroitin Sulfates |2 NLM | |
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650 | 7 | |a Collagen |2 NLM | |
650 | 7 | |a 9007-34-5 |2 NLM | |
700 | 1 | |a Lin, Lili |e verfasserin |4 aut | |
700 | 1 | |a Wen, Fuli |e verfasserin |4 aut | |
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