Low serum Kallistatin level was associated with poor neurological outcome of out-of-hospital cardiac arrest survivors : Proteomics study
Copyright © 2018 Elsevier B.V. All rights reserved..
AIM OF THE STUDY: To identify proteins of which depletion are associated with the poor 6-month neurological outcome of out-of-hospital cardiac arrest survivors.
METHODS: Seven healthy volunteers and 34 out-of-hospital cardiac arrest survivors admitted to the intensive care unit (ICU) and underwent targeted-temperature management were enrolled. According to the 6-month cerebral performance category (CPC) scale, patients were divided into the good (CPC 1-2) and poor (CPC 3-5) outcome groups. Blood samples were obtained at 0, 24, and 72 h after admission to the ICU.
RESULTS: With proteomic approaches, we found 23 proteins that showed group-differences between the sera pooled from 7 study groups: healthy volunteers, the good outcome groups (0, 24, and 72 h), and the poor outcome groups (0, 24, and 72 h). We selected 7 candidate proteins of which intensities were different between the good and poor outcome groups (>2-fold change) and excluded 5 proteins related to haemolysis or remaining high abundant proteins. To confirm the 2 identified proteins: retinal dehydrogenase 1 and Kallistatin, we performed enzyme-linked immunosorbent assay with individual serum. Finally, old age (odds ratio = 1.055; 95% confidence interval, 1.002-1.112; p = 0.043) and low serum kallistatin level at 0 h (odds ratio = 0.784; 95% confidence interval, 0.618-0.995; p = 0.046) were independently associated with the poor 6-month neurological outcome.
CONCLUSION: The depletion of serum kallistatin at admission to the ICU was associated with the poor neurological outcome of out-of-hospital cardiac arrest survivors.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2018 |
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| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:128 |
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| Enthalten in: |
Resuscitation - 128(2018) vom: 15. Juli, Seite 6-10 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jung, Yoon Sun [VerfasserIn] |
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| Links: |
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| Themen: |
Biomarkers |
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| Anmerkungen: |
Date Completed 18.12.2018 Date Revised 18.12.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.resuscitation.2018.04.025 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM283463740 |
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| 100 | 1 | |a Jung, Yoon Sun |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Low serum Kallistatin level was associated with poor neurological outcome of out-of-hospital cardiac arrest survivors |b Proteomics study |
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| 500 | |a Date Completed 18.12.2018 | ||
| 500 | |a Date Revised 18.12.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018 Elsevier B.V. All rights reserved. | ||
| 520 | |a AIM OF THE STUDY: To identify proteins of which depletion are associated with the poor 6-month neurological outcome of out-of-hospital cardiac arrest survivors | ||
| 520 | |a METHODS: Seven healthy volunteers and 34 out-of-hospital cardiac arrest survivors admitted to the intensive care unit (ICU) and underwent targeted-temperature management were enrolled. According to the 6-month cerebral performance category (CPC) scale, patients were divided into the good (CPC 1-2) and poor (CPC 3-5) outcome groups. Blood samples were obtained at 0, 24, and 72 h after admission to the ICU | ||
| 520 | |a RESULTS: With proteomic approaches, we found 23 proteins that showed group-differences between the sera pooled from 7 study groups: healthy volunteers, the good outcome groups (0, 24, and 72 h), and the poor outcome groups (0, 24, and 72 h). We selected 7 candidate proteins of which intensities were different between the good and poor outcome groups (>2-fold change) and excluded 5 proteins related to haemolysis or remaining high abundant proteins. To confirm the 2 identified proteins: retinal dehydrogenase 1 and Kallistatin, we performed enzyme-linked immunosorbent assay with individual serum. Finally, old age (odds ratio = 1.055; 95% confidence interval, 1.002-1.112; p = 0.043) and low serum kallistatin level at 0 h (odds ratio = 0.784; 95% confidence interval, 0.618-0.995; p = 0.046) were independently associated with the poor 6-month neurological outcome | ||
| 520 | |a CONCLUSION: The depletion of serum kallistatin at admission to the ICU was associated with the poor neurological outcome of out-of-hospital cardiac arrest survivors | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Heart arrest | |
| 650 | 4 | |a Hypothermia | |
| 650 | 4 | |a Induced | |
| 650 | 4 | |a Kallistatin | |
| 650 | 4 | |a Proteomics | |
| 650 | 7 | |a Biomarkers |2 NLM | |
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| 650 | 7 | |a kallistatin |2 NLM | |
| 650 | 7 | |a Retinal Dehydrogenase |2 NLM | |
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| 700 | 1 | |a Kwon, Woon Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Suh, Gil Joon |e verfasserin |4 aut | |
| 700 | 1 | |a Moon, Sungyoon |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Mi-Hee |e verfasserin |4 aut | |
| 700 | 1 | |a Youn, Je-In |e verfasserin |4 aut | |
| 700 | 1 | |a Seo, Sang-Uk |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Kyung Su |e verfasserin |4 aut | |
| 700 | 1 | |a Seong, Seung-Yong |e verfasserin |4 aut | |
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