Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chicken
Copyright © 2018 Elsevier Inc. All rights reserved..
An aptamer against Streptococcus pyogenes was selected and identified, and a fluorescent method based on the reported aptamer was established to detect S. pyogenes in the cooked chicken. Through a twelve rounds of whole-bacterium SELEX (systematic evolution of ligands by exponential enrichment) selection in vitro, a set of aptamers binding to the whole cell of S. pyogenes were generated, harvesting a low-level dissociation constant (Kd) value of 44 ± 5 nmol L-1 of aptamer S-12. Aptamer-based quantification of S. pyogenes in the cooked chicken sample was implemented in a fluorescence resonance energy transfer-based assay by using graphene oxide, resulting in a limit of detection of 70 cfu mL-1. The selected aptamer showed affinity and selectivity recognizing S. pyogenes; besides, more biosensors based on the selected aptamer as a molecular recognition element could be developed in the innovative determinations of S. pyogenes.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2018 |
|---|---|
| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:551 |
|---|---|
| Enthalten in: |
Analytical biochemistry - 551(2018) vom: 15. Juni, Seite 37-42 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Huang, Yukun [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
7782-42-5 |
|---|
| Anmerkungen: |
Date Completed 22.07.2019 Date Revised 22.07.2019 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.ab.2018.04.015 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM283462965 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM283462965 | ||
| 003 | DE-627 | ||
| 005 | 20231225040927.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ab.2018.04.015 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0944.xml |
| 035 | |a (DE-627)NLM283462965 | ||
| 035 | |a (NLM)29698672 | ||
| 035 | |a (PII)S0003-2697(18)30209-4 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Huang, Yukun |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Selection and characterization, application of a DNA aptamer targeted to Streptococcus pyogenes in cooked chicken |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.07.2019 | ||
| 500 | |a Date Revised 22.07.2019 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2018 Elsevier Inc. All rights reserved. | ||
| 520 | |a An aptamer against Streptococcus pyogenes was selected and identified, and a fluorescent method based on the reported aptamer was established to detect S. pyogenes in the cooked chicken. Through a twelve rounds of whole-bacterium SELEX (systematic evolution of ligands by exponential enrichment) selection in vitro, a set of aptamers binding to the whole cell of S. pyogenes were generated, harvesting a low-level dissociation constant (Kd) value of 44 ± 5 nmol L-1 of aptamer S-12. Aptamer-based quantification of S. pyogenes in the cooked chicken sample was implemented in a fluorescence resonance energy transfer-based assay by using graphene oxide, resulting in a limit of detection of 70 cfu mL-1. The selected aptamer showed affinity and selectivity recognizing S. pyogenes; besides, more biosensors based on the selected aptamer as a molecular recognition element could be developed in the innovative determinations of S. pyogenes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Aptamer | |
| 650 | 4 | |a Cooked chicken | |
| 650 | 4 | |a Graphene oxide | |
| 650 | 4 | |a Streptococcus pyogenes | |
| 650 | 4 | |a Whole-bacterium SELEX | |
| 650 | 7 | |a Aptamers, Nucleotide |2 NLM | |
| 650 | 7 | |a DNA, Bacterial |2 NLM | |
| 650 | 7 | |a graphene oxide |2 NLM | |
| 650 | 7 | |a Graphite |2 NLM | |
| 650 | 7 | |a 7782-42-5 |2 NLM | |
| 700 | 1 | |a Wang, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Duan, Nuo |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zhouping |e verfasserin |4 aut | |
| 700 | 1 | |a Che, Zhenming |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Lijun |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Xiao |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xianggui |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Analytical biochemistry |d 1960 |g 551(2018) vom: 15. Juni, Seite 37-42 |w (DE-627)NLM000012556 |x 1096-0309 |7 nnns |
| 773 | 1 | 8 | |g volume:551 |g year:2018 |g day:15 |g month:06 |g pages:37-42 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.ab.2018.04.015 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 551 |j 2018 |b 15 |c 06 |h 37-42 | ||