Metabolomics reveals perturbations in endometrium and serum of minimal and mild endometriosis

Endometriosis is a common benign gynecological disease, characterized by growth and proliferation of endometrial glands and stroma outside the uterus. With studies showing metabolic changes in various biofluids of endometriosis women, we have set upon to investigate whether endometrial tissue show differences in their metabolic profiles. 1H NMR analysis was performed on eutopic endometrial tissue of women with endometriosis and controls. Analysis was performed on spectral data and on relative concentrations of metabolites obtained from spectra using multivariate and univariate data analysis. Analysis shows that various energy, ketogenic and glucogenic metabolites have significant altered concentrations in various stages of endometriosis. In addition, altered tissue metabolites in minimal and mild stages of endometriosis were explored in serum of these patients to assess their role in disease diagnosis. For Stage I diagnosis alanine was found to have 90% sensitivity (true positives) and 58% specificity (true negatives). For Stage II diagnosis alanine, leucine, lysine, proline and phenylalanine showed significant altered levels in serum. While sensitivity of these serum metabolites varied between 69.2-100% the specificity values ranged between 58.3-91.7%. Further, a regression model generated with this panel of serum markers showed an improved sensitivity and specificity of 100% and 83%, respectively for Stage II diagnosis.

Errataetall:

ErratumIn: Sci Rep. 2020 Feb 12;10(1):2790. - PMID 32051518

Medienart:

E-Artikel

Erscheinungsjahr:

2018

Erschienen:

2018

Enthalten in:

Zur Gesamtaufnahme - volume:8

Enthalten in:

Scientific reports - 8(2018), 1 vom: 24. Apr., Seite 6466

Sprache:

Englisch

Beteiligte Personen:

Dutta, Mainak [VerfasserIn]
Singh, Brajesh [VerfasserIn]
Joshi, Mamata [VerfasserIn]
Das, Debanjan [VerfasserIn]
Subramani, Elavarasan [VerfasserIn]
Maan, Meenu [VerfasserIn]
Jana, Saikat Kumar [VerfasserIn]
Sharma, Uma [VerfasserIn]
Das, Soumen [VerfasserIn]
Dasgupta, Swagata [VerfasserIn]
Ray, Chaitali Datta [VerfasserIn]
Chakravarty, Baidyanath [VerfasserIn]
Chaudhury, Koel [VerfasserIn]

Links:

Volltext

Themen:

Biomarkers
Journal Article
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 04.11.2019

Date Revised 06.03.2020

published: Electronic

ErratumIn: Sci Rep. 2020 Feb 12;10(1):2790. - PMID 32051518

Citation Status MEDLINE

doi:

10.1038/s41598-018-23954-7

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM28339207X