Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation
Multiplex immunoassays are used for rapid profiling of biomarker proteins and small molecules in biological fluids. The advantages over single immunoassays include lower sample consumption, cost, and labor. This chapter details a protocol to develop a 5-plex assay for glucagon-like peptide 1, growth hormone, insulin, leptin, and thyroid-stimulating hormone on the Luminex® platform. The results of the analysis of insulin in normal control subjects are given due to the important role of this hormone in nutritional programming diseases.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2018 |
|---|---|
| Erschienen: |
2018 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1735 |
|---|---|
| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 1735(2018) vom: 31., Seite 449-456 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Stephen, Laurie [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antibody |
|---|
| Anmerkungen: |
Date Completed 31.12.2018 Date Revised 31.12.2018 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1007/978-1-4939-7614-0_32 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM280405545 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM280405545 | ||
| 003 | DE-627 | ||
| 005 | 20231225025246.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/978-1-4939-7614-0_32 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0934.xml |
| 035 | |a (DE-627)NLM280405545 | ||
| 035 | |a (NLM)29380335 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Stephen, Laurie |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation |
| 264 | 1 | |c 2018 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 31.12.2018 | ||
| 500 | |a Date Revised 31.12.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Multiplex immunoassays are used for rapid profiling of biomarker proteins and small molecules in biological fluids. The advantages over single immunoassays include lower sample consumption, cost, and labor. This chapter details a protocol to develop a 5-plex assay for glucagon-like peptide 1, growth hormone, insulin, leptin, and thyroid-stimulating hormone on the Luminex® platform. The results of the analysis of insulin in normal control subjects are given due to the important role of this hormone in nutritional programming diseases | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antibody | |
| 650 | 4 | |a Biomarker | |
| 650 | 4 | |a Disease | |
| 650 | 4 | |a Hormone | |
| 650 | 4 | |a Insulin | |
| 650 | 4 | |a Luminex assay | |
| 650 | 4 | |a Multiplex assay | |
| 650 | 4 | |a Nutritional programming | |
| 650 | 7 | |a Biomarkers |2 NLM | |
| 650 | 7 | |a Hormones |2 NLM | |
| 700 | 1 | |a Guest, Paul C |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Methods in molecular biology (Clifton, N.J.) |d 1984 |g 1735(2018) vom: 31., Seite 449-456 |w (DE-627)NLM074849794 |x 1940-6029 |7 nnns |
| 773 | 1 | 8 | |g volume:1735 |g year:2018 |g day:31 |g pages:449-456 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/978-1-4939-7614-0_32 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 1735 |j 2018 |b 31 |h 449-456 | ||