A heterogeneous Ly-6B2+ leukocyte population consists of yet undescribed iNOS-expressing cell types in murine skin wounds

Copyright © 2018 Elsevier Inc. All rights reserved..

The gaseous mediator nitric oxide (NO) is a central regulatory molecule during the inflammatory phase of cutaneous tissue repair. The inducible NO-synthase (iNOS) represents the main isoform of the three NO producing enzymes at the wound site. In particular, keratinocytes and macrophages are described as main sources of iNOS-derived NO in skin wounds. Here we provide experimental evidence that Ly-6B2+ leukocytes are an additional cellular source of iNOS-derived NO in wounds. As wound iNOS protein expression temporally coincides with both macrophage and neutrophil infiltration, we used immunohistochemistry (IHC) and fluorescence-activated cell sorting (FACS) to address iNOS expression in both macrophages and neutrophil subsets. IHC analyses excluded F4/80+ macrophages as iNOS producers, but indicated Ly-6G/C (Gr-1)+ neutrophils to express iNOS in wound granulation tissue. A subsequent FACS-based analysis from cellular wound tissue preparations revealed an iNOS-expressing fraction of Ly-6B2-determined leukocytes that consisted of Ly-6G+ and Ly-6G- cells, meaning that mainly mature neutrophils (Ly-6B2+/Ly-6G+) as well as inflammatory monocytes (Ly-6B2+/Ly-6G-) are dominant iNOS-expressing cell types in the developing granulation tissue of acute wounds.

Medienart:

E-Artikel

Erscheinungsjahr:

2018

Erschienen:

2018

Enthalten in:

Zur Gesamtaufnahme - volume:74

Enthalten in:

Nitric oxide : biology and chemistry - 74(2018) vom: 01. Apr., Seite 23-31

Sprache:

Englisch

Beteiligte Personen:

Goren, Itamar [VerfasserIn]
Christen, Urs [VerfasserIn]
Pfeilschifter, Josef [VerfasserIn]
Frank, Stefan [VerfasserIn]

Links:

Volltext

Themen:

Acute wound
Antigens, Ly
EC 1.14.13.39
Gene expression
Journal Article
Macrophages
Neutrophils
Nitric Oxide Synthase Type II
Nitric oxide
Wound healing

Anmerkungen:

Date Completed 01.04.2019

Date Revised 01.04.2019

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.niox.2018.01.004

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM280175086