Induction by innate defence regulator peptide 1018 of pro-angiogenic molecules and endothelial cell migration in a high glucose environment

Copyright © 2018 Elsevier Inc. All rights reserved..

Synthetic innate defence regulator (IDR) peptides such as IDR-1018 modulate immunity to promote key protective functions including chemotaxis, wound healing, and anti-infective activity, while suppressing pro-inflammatory responses to non-pathological levels. Here we demonstrated that IDR-1018 induced, by up to 75-fold, pro-angiogenic VEGF-165 in keratinocytes but suppressed this isoform in endothelial cells. It also induced early angiogenin and prolonged anti-inflammatory TGFβ expression on endothelial cells, while suppressing early pro-inflammatory IL-1β expression levels. IDR-1018 also down-regulated the hypoxia induced transcription factor HIF-1α in both keratinocytes and endothelial cells. Consistent with these data, in an in vitro wound healing scratch assay, IDR-1018 induced migration of endothelial cells under conditions of hypoxia while in epithelial cells migration increased only under conditions of normoxia.

Medienart:

E-Artikel

Erscheinungsjahr:

2018

Erschienen:

2018

Enthalten in:

Zur Gesamtaufnahme - volume:101

Enthalten in:

Peptides - 101(2018) vom: 06. März, Seite 135-144

Sprache:

Englisch

Beteiligte Personen:

Marin-Luevano, Paulina [VerfasserIn]
Trujillo, Valentin [VerfasserIn]
Rodriguez-Carlos, Adrian [VerfasserIn]
González-Curiel, Irma [VerfasserIn]
Enciso-Moreno, Jose A [VerfasserIn]
Hancock, Robert E W [VerfasserIn]
Rivas-Santiago, Bruno [VerfasserIn]

Links:

Volltext

Themen:

Angiogenesis
Angiogenesis Inducing Agents
Angiogenin
Antimicrobial Cationic Peptides
Diabetic foot ulcer
Glucose
HIF1A protein, human
Hypoxia-Inducible Factor 1, alpha Subunit
IDR-1018
IL1B protein, human
IY9XDZ35W2
Immunologic Factors
Innate defense regulating peptide 1018
Interleukin-1beta
Journal Article
Research Support, Non-U.S. Gov't
VEGFA protein, human
VGF
Vascular Endothelial Growth Factor A
Wound healing

Anmerkungen:

Date Completed 26.12.2018

Date Revised 26.12.2018

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.peptides.2018.01.010

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM280147732