Peptide-Based Photoelectrochemical Cytosensor Using a Hollow-TiO2/EG/ZnIn2S4 Cosensitized Structure for Ultrasensitive Detection of Early Apoptotic Cells and Drug Evaluation

The ability to rapidly detect apoptotic cells and accurately evaluate therapeutic effects is significant in cancer research. To address this target, a biocompatible, ultrasensitive photoelectrochemical (PEC) cytosensing platform was developed based on electrochemically reduced graphene (EG)/ZnIn2S4 cosensitized TiO2 coupled with specific recognition between apoptotic cells and phosphatidylserine-binding peptide (PSBP). In this strategy, the HL-60 cells were selected as a model and C005, nilotinib, and imatinib were selected as apoptosis inducers to show cytosensing performances. In particular, a TiO2 photoactive substrate was designed as hollow spheres to enhance the PEC performance. Graphene was electrodeposited on the hollow TiO2-modified electrode to accelerate electron transfer and increase conductivity, followed by in situ growth of ZnIn2S4 nanocrystals as photosensitizers via successive ionic layer adsorption and reaction method, forming a TiO2/EG/ZnIn2S4 cosensitized structure that was used as a PEC matrix to immobilize PSBP for the recognition of early apoptotic cells. The detection of apoptotic cells was based on steric hindrance originating from apoptotic cell capture to induce an obvious decrease in the photocurrent signal. The ultrahigh sensitivity of the cytosensor resulted from enhanced PEC performance, bioactivity, and high binding affinity between PSBP and apoptotic cells. Compared with other assays, incorporate toxic elements were avoided, such as Cd, Ru, and Te, which ensured normal cell growth and are appropriate for cell analysis. The designed PEC cytosensor showed a low detection limit of apoptotic cells (as low as three cells), a wide linear range from 1 × 103 to 5 × 107 cells/mL, and an accurate evaluation of therapeutic effects. It also exhibited good specificity, reproducibility, and stability.

Medienart:

E-Artikel

Erscheinungsjahr:

2018

Erschienen:

2018

Enthalten in:

Zur Gesamtaufnahme - volume:10

Enthalten in:

ACS applied materials & interfaces - 10(2018), 5 vom: 07. Feb., Seite 4429-4438

Sprache:

Englisch

Beteiligte Personen:

Wu, Rong [VerfasserIn]
Fan, Gao-Chao [VerfasserIn]
Jiang, Li-Ping [VerfasserIn]
Zhu, Jun-Jie [VerfasserIn]

Links:

Volltext

Themen:

15FIX9V2JP
7782-42-5
Apoptotic cell
Cadmium Compounds
Cosensitized
Cytosensing
D1JT611TNE
Drug evaluation
Graphite
Journal Article
Peptides
Photoelectrochemistry
Titanium
Titanium dioxide

Anmerkungen:

Date Completed 12.02.2019

Date Revised 15.02.2019

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1021/acsami.7b16054

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM279902875