Geographic clonal tracking in macaques provides insights into HSPC migration and differentiation

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The geographic distribution of hematopoiesis at a clonal level is of interest in understanding how hematopoietic stem and progenitor cells (HSPCs) and their progeny interact with bone marrow (BM) niches during regeneration. We tagged rhesus macaque autologous HSPCs with genetic barcodes, allowing clonal tracking over time and space after transplantation. We found marked geographic segregation of CD34+ HSPCs for at least 6 mo posttransplantation, followed by very gradual clonal mixing at different BM sites over subsequent months to years. Clonal mapping was used to document local production of granulocytes, monocytes, B cells, and CD56+ natural killer (NK) cells. In contrast, CD16+CD56- NK cells were not produced in the BM, and in fact were clonally distinct from multipotent progenitors producing all other lineages. Most surprisingly, we documented local BM production of CD3+ T cells early after transplantation, using both clonal mapping and intravascular versus tissue-resident T cell staining, suggesting a thymus-independent T cell developmental pathway operating during BM regeneration, perhaps before thymic recovery.

Errataetall:

CommentIn: J Exp Med. 2018 Jan 2;215(1):13-15. - PMID 29183989

Medienart:

E-Artikel

Erscheinungsjahr:

2018

Erschienen:

2018

Enthalten in:

Zur Gesamtaufnahme - volume:215

Enthalten in:

The Journal of experimental medicine - 215(2018), 1 vom: 02. Jan., Seite 217-232

Sprache:

Englisch

Beteiligte Personen:

Wu, Chuanfeng [VerfasserIn]
Espinoza, Diego A [VerfasserIn]
Koelle, Samson J [VerfasserIn]
Potter, E Lake [VerfasserIn]
Lu, Rong [VerfasserIn]
Li, Brian [VerfasserIn]
Yang, Di [VerfasserIn]
Fan, Xing [VerfasserIn]
Donahue, Robert E [VerfasserIn]
Roederer, Mario [VerfasserIn]
Dunbar, Cynthia E [VerfasserIn]

Links:

Volltext

Themen:

Biomarkers
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 01.01.2019

Date Revised 01.01.2019

published: Print-Electronic

CommentIn: J Exp Med. 2018 Jan 2;215(1):13-15. - PMID 29183989

Citation Status MEDLINE

doi:

10.1084/jem.20171341

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM278083161