Geographic clonal tracking in macaques provides insights into HSPC migration and differentiation
This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply..
The geographic distribution of hematopoiesis at a clonal level is of interest in understanding how hematopoietic stem and progenitor cells (HSPCs) and their progeny interact with bone marrow (BM) niches during regeneration. We tagged rhesus macaque autologous HSPCs with genetic barcodes, allowing clonal tracking over time and space after transplantation. We found marked geographic segregation of CD34+ HSPCs for at least 6 mo posttransplantation, followed by very gradual clonal mixing at different BM sites over subsequent months to years. Clonal mapping was used to document local production of granulocytes, monocytes, B cells, and CD56+ natural killer (NK) cells. In contrast, CD16+CD56- NK cells were not produced in the BM, and in fact were clonally distinct from multipotent progenitors producing all other lineages. Most surprisingly, we documented local BM production of CD3+ T cells early after transplantation, using both clonal mapping and intravascular versus tissue-resident T cell staining, suggesting a thymus-independent T cell developmental pathway operating during BM regeneration, perhaps before thymic recovery.
Errataetall: |
CommentIn: J Exp Med. 2018 Jan 2;215(1):13-15. - PMID 29183989 |
---|---|
Medienart: |
E-Artikel |
Erscheinungsjahr: |
2018 |
---|---|
Erschienen: |
2018 |
Enthalten in: |
Zur Gesamtaufnahme - volume:215 |
---|---|
Enthalten in: |
The Journal of experimental medicine - 215(2018), 1 vom: 02. Jan., Seite 217-232 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Wu, Chuanfeng [VerfasserIn] |
---|
Links: |
---|
Themen: |
Biomarkers |
---|
Anmerkungen: |
Date Completed 01.01.2019 Date Revised 01.01.2019 published: Print-Electronic CommentIn: J Exp Med. 2018 Jan 2;215(1):13-15. - PMID 29183989 Citation Status MEDLINE |
---|
doi: |
10.1084/jem.20171341 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM278083161 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM278083161 | ||
003 | DE-627 | ||
005 | 20231225015738.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2018 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1084/jem.20171341 |2 doi | |
028 | 5 | 2 | |a pubmed24n0926.xml |
035 | |a (DE-627)NLM278083161 | ||
035 | |a (NLM)29141868 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Wu, Chuanfeng |e verfasserin |4 aut | |
245 | 1 | 0 | |a Geographic clonal tracking in macaques provides insights into HSPC migration and differentiation |
264 | 1 | |c 2018 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 01.01.2019 | ||
500 | |a Date Revised 01.01.2019 | ||
500 | |a published: Print-Electronic | ||
500 | |a CommentIn: J Exp Med. 2018 Jan 2;215(1):13-15. - PMID 29183989 | ||
500 | |a Citation Status MEDLINE | ||
520 | |a This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply. | ||
520 | |a The geographic distribution of hematopoiesis at a clonal level is of interest in understanding how hematopoietic stem and progenitor cells (HSPCs) and their progeny interact with bone marrow (BM) niches during regeneration. We tagged rhesus macaque autologous HSPCs with genetic barcodes, allowing clonal tracking over time and space after transplantation. We found marked geographic segregation of CD34+ HSPCs for at least 6 mo posttransplantation, followed by very gradual clonal mixing at different BM sites over subsequent months to years. Clonal mapping was used to document local production of granulocytes, monocytes, B cells, and CD56+ natural killer (NK) cells. In contrast, CD16+CD56- NK cells were not produced in the BM, and in fact were clonally distinct from multipotent progenitors producing all other lineages. Most surprisingly, we documented local BM production of CD3+ T cells early after transplantation, using both clonal mapping and intravascular versus tissue-resident T cell staining, suggesting a thymus-independent T cell developmental pathway operating during BM regeneration, perhaps before thymic recovery | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, N.I.H., Extramural | |
650 | 4 | |a Research Support, N.I.H., Intramural | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a Biomarkers |2 NLM | |
700 | 1 | |a Espinoza, Diego A |e verfasserin |4 aut | |
700 | 1 | |a Koelle, Samson J |e verfasserin |4 aut | |
700 | 1 | |a Potter, E Lake |e verfasserin |4 aut | |
700 | 1 | |a Lu, Rong |e verfasserin |4 aut | |
700 | 1 | |a Li, Brian |e verfasserin |4 aut | |
700 | 1 | |a Yang, Di |e verfasserin |4 aut | |
700 | 1 | |a Fan, Xing |e verfasserin |4 aut | |
700 | 1 | |a Donahue, Robert E |e verfasserin |4 aut | |
700 | 1 | |a Roederer, Mario |e verfasserin |4 aut | |
700 | 1 | |a Dunbar, Cynthia E |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t The Journal of experimental medicine |d 1896 |g 215(2018), 1 vom: 02. Jan., Seite 217-232 |w (DE-627)NLM000005967 |x 1540-9538 |7 nnns |
773 | 1 | 8 | |g volume:215 |g year:2018 |g number:1 |g day:02 |g month:01 |g pages:217-232 |
856 | 4 | 0 | |u http://dx.doi.org/10.1084/jem.20171341 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 215 |j 2018 |e 1 |b 02 |c 01 |h 217-232 |