Overexpression of Recombinant Selenoproteins in E. coli

Expression of selenoproteins necessitates a process of decoding of a UGA codon from termination of translation to insertion of selenocysteine. The mechanisms of this process pose major challenges with regards to recombinant selenoprotein production in E. coli, which however can be overcome especially if the Sec residue is located close to the C-terminal end, as is the case for several naturally found selenoproteins. This chapter summarizes a method to achieve such a production.

Medienart:

E-Artikel

Erscheinungsjahr:

2018

Erschienen:

2018

Enthalten in:

Zur Gesamtaufnahme - volume:1661

Enthalten in:

Methods in molecular biology (Clifton, N.J.) - 1661(2018) vom: 15., Seite 231-240

Sprache:

Englisch

Beteiligte Personen:

Cheng, Qing [VerfasserIn]
Arnér, Elias S J [VerfasserIn]

Links:

Volltext

Themen:

0CH9049VIS
3' Untranslated Regions
Bacterial Proteins
Codon, Terminator
Journal Article
RF2 (prfB)
RNA, Messenger
Recombinant
Recombinant Proteins
SECIS element
SelB
SelB protein, Bacteria
Selenocysteine
Selenoprotein
Selenoproteins
TGR
Thioredoxin reductase

Anmerkungen:

Date Completed 21.05.2018

Date Revised 09.12.2020

published: Print

Citation Status MEDLINE

doi:

10.1007/978-1-4939-7258-6_17

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM275877213