Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates
© FEMS 2017..
Soluble methane monooxygenase (sMMO) from methane-oxidising bacteria can oxygenate more than 100 hydrocarbons and is one of the most catalytically versatile biological oxidation catalysts. Expression of recombinant sMMO has to date not been achieved in Escherichia coli and so an alternative expression system must be used to manipulate it genetically. Here we report substantial improvements to the previously described system for mutagenesis of sMMO and expression of recombinant enzymes in a methanotroph (Methylosinus trichosporium OB3b) expression system. This system has been utilised to make a number of new mutants and to engineer sMMO to increase its catalytic precision with a specific substrate whilst increasing activity by up to 6-fold. These results are the first 'proof-of-principle' experiments illustrating the feasibility of developing sMMO-derived catalysts for diverse applications.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2017 |
|---|---|
| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:364 |
|---|---|
| Enthalten in: |
FEMS microbiology letters - 364(2017), 13 vom: 06. Juli |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Lock, Malcolm [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Biocatalysis |
|---|
| Anmerkungen: |
Date Completed 20.04.2018 Date Revised 15.03.2024 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1093/femsle/fnx137 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM275270521 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM275270521 | ||
| 003 | DE-627 | ||
| 005 | 20240315232407.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1093/femsle/fnx137 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1330.xml |
| 035 | |a (DE-627)NLM275270521 | ||
| 035 | |a (NLM)28854685 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Lock, Malcolm |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Mutagenesis and expression of methane monooxygenase to alter regioselectivity with aromatic substrates |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 20.04.2018 | ||
| 500 | |a Date Revised 15.03.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © FEMS 2017. | ||
| 520 | |a Soluble methane monooxygenase (sMMO) from methane-oxidising bacteria can oxygenate more than 100 hydrocarbons and is one of the most catalytically versatile biological oxidation catalysts. Expression of recombinant sMMO has to date not been achieved in Escherichia coli and so an alternative expression system must be used to manipulate it genetically. Here we report substantial improvements to the previously described system for mutagenesis of sMMO and expression of recombinant enzymes in a methanotroph (Methylosinus trichosporium OB3b) expression system. This system has been utilised to make a number of new mutants and to engineer sMMO to increase its catalytic precision with a specific substrate whilst increasing activity by up to 6-fold. These results are the first 'proof-of-principle' experiments illustrating the feasibility of developing sMMO-derived catalysts for diverse applications | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a biocatalysis | |
| 650 | 4 | |a hydrocarbon oxidation | |
| 650 | 4 | |a methane | |
| 650 | 4 | |a monooxygenase | |
| 650 | 4 | |a protein engineering | |
| 650 | 7 | |a Recombinant Proteins |2 NLM | |
| 650 | 7 | |a Oxygenases |2 NLM | |
| 650 | 7 | |a EC 1.13.- |2 NLM | |
| 650 | 7 | |a methane monooxygenase |2 NLM | |
| 650 | 7 | |a EC 1.14.13.25 |2 NLM | |
| 650 | 7 | |a Methane |2 NLM | |
| 650 | 7 | |a OP0UW79H66 |2 NLM | |
| 700 | 1 | |a Nichol, Tim |e verfasserin |4 aut | |
| 700 | 1 | |a Murrell, J Colin |e verfasserin |4 aut | |
| 700 | 1 | |a Smith, Thomas J |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t FEMS microbiology letters |d 1984 |g 364(2017), 13 vom: 06. Juli |w (DE-627)NLM012613770 |x 1574-6968 |7 nnns |
| 773 | 1 | 8 | |g volume:364 |g year:2017 |g number:13 |g day:06 |g month:07 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1093/femsle/fnx137 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 364 |j 2017 |e 13 |b 06 |c 07 | ||