Clathrin Heavy Chain Subunits Coordinate Endo- and Exocytic Traffic and Affect Stomatal Movement

© 2017 The author(s). All Rights Reserved..

The current model for vesicular traffic to and from the plasma membrane is accepted, but the molecular requirements for this coordination are not well defined. We have identified the hot ABA-deficiency suppressor1 mutant, which has a stomatal function defect, as a clathrin heavy chain1 (CHC1) mutant allele and show that it has a decreased rate of endocytosis and growth defects that are shared with other chc1 mutant alleles. We used chc1 alleles and the related chc2 mutant as tools to investigate the effects that clathrin defects have on secretion pathways and plant growth. We show that secretion and endocytosis at the plasma membrane are sensitive to CHC1 and CHC2 function in seedling roots and that chc mutants have physiological defects in stomatal function and plant growth that have not been described previously. These findings suggest that clathrin supports specific functions in multiple cell types. Stomata movement and gas exchange are altered in chc mutants, indicating that clathrin is important for stomatal regulation. The aberrant function of chc mutant stomata is consistent with the growth phenotypes observed under different water and light conditions, which also are similar to those of the secretory SNARE mutant, syp121 The syp121 and chc mutants have impaired endocytosis and exocytosis compared with the wild type, indicating a link between SYP121-dependent secretion and clathrin-dependent endocytosis at the plasma membrane. Our findings provide evidence that clathrin and SYP121 functions are important for the coordination of endocytosis and exocytosis and have an impact on stomatal function, gas exchange, and vegetative growth in Arabidopsis (Arabidopsis thaliana).

Medienart:

E-Artikel

Erscheinungsjahr:

2017

Erschienen:

2017

Enthalten in:

Zur Gesamtaufnahme - volume:175

Enthalten in:

Plant physiology - 175(2017), 2 vom: 01. Okt., Seite 708-720

Sprache:

Englisch

Beteiligte Personen:

Larson, Emily R [VerfasserIn]
Van Zelm, Eva [VerfasserIn]
Roux, Camille [VerfasserIn]
Marion-Poll, Annie [VerfasserIn]
Blatt, Michael R [VerfasserIn]

Links:

Volltext

Themen:

114899-12-6
Arabidopsis Proteins
CHC1 protein, Arabidopsis
CHC2 protein, Arabidopsis
Clathrin Heavy Chains
Journal Article
PEN1 protein, Arabidopsis
Qa-SNARE Proteins

Anmerkungen:

Date Completed 13.06.2018

Date Revised 12.11.2023

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1104/pp.17.00970

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM275039137