Hypericin cytotoxicity in tumor and non-tumor cell lines : A chemometric study

Copyright © 2017 Elsevier B.V. All rights reserved..

Hypericin (HY) is an excellent photoactive compound that has been investigated for the photodynamic treatment of cancer as well as for microorganism inactivation. In this study, chemometric analysis was applied for the first time on photodynamic assays to investigate the cytotoxicity of HY in tumor (HEp-2) and non-tumor (Vero and HUVEC) cell lines. The experimental planning was based on eight assays using the 23 full factorial design combining three important variables for PDT: photosensitizer concentrations, incubation time of cells in HY solutions and employed light dose (λ=590±10nm). The statistical data analysis evidenced the relative significance of such variables and the correlations among them on the cell death. The chemometric results suggested that long incubation time and a low HY concentration and/or light dose allow killing selectively tumor cells. The chemometric analysis could be a new useful empiric method to a previous prediction of the IC50. In this study, the estimated values were in agreement with the experimental IC50 values.

Medienart:

E-Artikel

Erscheinungsjahr:

2017

Erschienen:

2017

Enthalten in:

Zur Gesamtaufnahme - volume:20

Enthalten in:

Photodiagnosis and photodynamic therapy - 20(2017) vom: 10. Dez., Seite 86-90

Sprache:

Englisch

Beteiligte Personen:

Gonçalves, Joyce Laura da Silva [VerfasserIn]
Bernal, Claudia [VerfasserIn]
Imasato, Hidetake [VerfasserIn]
Perussi, Janice Rodrigues [VerfasserIn]

Links:

Volltext

Themen:

5QD5427UN7
7V2F1075HD
Anthracenes
Chemometric analysis
Hypericin
IC(50) prediction model
Journal Article
Perylene
Photodynamic therapy
Photosensitizing Agents

Anmerkungen:

Date Completed 06.08.2018

Date Revised 04.12.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.pdpdt.2017.08.005

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM274845229