A tetracycline-inducible integrative expression system for Streptococcus pneumoniae
© FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
Inducible gene expression systems are very useful to analyze cellular processes. The ability to switch the expression state of genes of interest may even be crucial if essential traits or genetic instability are involved. An integrative plasmid, pTEX2, was designed using the (anhydro)tetracycline-inducible promoter Pxyl/tet from staphylococcal plasmid pRAB11 to control gene expression in Streptococcus pneumoniae. The system was evaluated by expressing genes of the two-component regulatory system ciaRH of S. pneumoniae. With full induction of Pxyl/tet, wild-type levels of the response regulator CiaR were obtained, while the uninduced basal expression was low. Hyperactive variants of the kinase gene ciaH normally causing pronounced genetic instability could be handled without any problems upon cloning into pTEX2. Therefore, the expression system is well suited to express physiological levels of proteins in S. pneumoniae and also to aid regulatory studies.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:364 |
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| Enthalten in: |
FEMS microbiology letters - 364(2017), 5 vom: 01. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Meiers, Marina [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 20.11.2017 Date Revised 23.02.2018 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/femsle/fnx044 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 2 | |a A tetracycline-inducible integrative expression system for Streptococcus pneumoniae |
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| 500 | |a Date Revised 23.02.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a Inducible gene expression systems are very useful to analyze cellular processes. The ability to switch the expression state of genes of interest may even be crucial if essential traits or genetic instability are involved. An integrative plasmid, pTEX2, was designed using the (anhydro)tetracycline-inducible promoter Pxyl/tet from staphylococcal plasmid pRAB11 to control gene expression in Streptococcus pneumoniae. The system was evaluated by expressing genes of the two-component regulatory system ciaRH of S. pneumoniae. With full induction of Pxyl/tet, wild-type levels of the response regulator CiaR were obtained, while the uninduced basal expression was low. Hyperactive variants of the kinase gene ciaH normally causing pronounced genetic instability could be handled without any problems upon cloning into pTEX2. Therefore, the expression system is well suited to express physiological levels of proteins in S. pneumoniae and also to aid regulatory studies | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Streptococcus pneumoniae | |
| 650 | 4 | |a integrative plasmid | |
| 650 | 4 | |a tetracycline-inducible expression | |
| 650 | 4 | |a two-component regulatory system CiaRH | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Tetracycline |2 NLM | |
| 650 | 7 | |a F8VB5M810T |2 NLM | |
| 700 | 1 | |a Laux, Anke |e verfasserin |4 aut | |
| 700 | 1 | |a Eichinger, Daniela |e verfasserin |4 aut | |
| 700 | 1 | |a Sexauer, Anne |e verfasserin |4 aut | |
| 700 | 1 | |a Marx, Patrick |e verfasserin |4 aut | |
| 700 | 1 | |a Bertram, Ralph |e verfasserin |4 aut | |
| 700 | 1 | |a Brückner, Reinhold |e verfasserin |4 aut | |
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