Ionic liquid-assisted separation and determination of selenium species in food and beverage samples by liquid chromatography coupled to hydride generation atomic fluorescence spectrometry

Copyright © 2017 Elsevier B.V. All rights reserved..

Different ionic liquids (ILs) were assayed as mobile phase modifiers for the separation and determination of selenite [Se(IV)], selenate [Se(VI)], selenomethionine (SeMet) and Se-methylselenocysteine (SeMeSeCys) by reversed-phase high-performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry (RP-HPLC-HG-AFS). The use of several ILs: 1-butyl-3-methylimidazolium chloride, 1-hexyl-3-methylimidazolium chloride ([C6mim]Cl), 1-octyl-3-methylimidazolium chloride, 1-dodecyl-3-methylimidazolium bromide, 1-hexadecyl-3-methylimidazolium bromide and tributyl(methyl)phosphonium methylsulphate was evaluated. Also, the effect of pH, buffer type and IL concentration on the separation of Se species was studied. Complete separation was attained within 12min using a C8 column and a gradient performed with a mobile phase containing 0.1% (v/v) [C6mim]Cl at pH 6.0. The proposed method allows the separation of inorganic and organic Se species in a single chromatographic run, adding further benefits over already reported methods based on RP-HPLC. In addition, the influence of ILs on the AFS signals of each Se species was evaluated and a multivariate methodology was used for optimization of AFS sensitivity. The limits of detection were 0.92, 0.86, 1.41 and 1.19μgL-1 for Se(IV), Se(VI), SeMet and SeMeSeCys, respectively. The method was successfully applied for speciation analysis of Se in complex samples, such as wine, beer, yeast and garlic.

Medienart:

E-Artikel

Erscheinungsjahr:

2017

Erschienen:

2017

Enthalten in:

Zur Gesamtaufnahme - volume:1491

Enthalten in:

Journal of chromatography. A - 1491(2017) vom: 31. März, Seite 117-125

Sprache:

Englisch

Beteiligte Personen:

Castro Grijalba, Alexander [VerfasserIn]
Fiorentini, Emiliano F [VerfasserIn]
Wuilloud, Rodolfo G [VerfasserIn]

Links:

Volltext

Themen:

Atomic fluorescence spectroscopy
Beverage
Food
H6241UJ22B
Ionic Liquids
Ionic liquid
Journal Article
Organoselenium Compounds
Reversed-phase chromatography
Selenium
Selenium species

Anmerkungen:

Date Completed 16.05.2017

Date Revised 02.12.2018

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.chroma.2017.02.045

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM269414835