Measuring Oxygen Consumption Rate in Caenorhabditis elegans
The rate of oxygen consumption is a vital marker indicating cellular function during lifetime under normal or metabolically challenged conditions. It is used broadly to study mitochondrial function (Artal-Sanz and Tavernarakis, 2009; Palikaras et al., 2015; Ryu et al., 2016) or investigate factors mediating the switch from oxidative phosphorylation to aerobic glycolysis (Chen et al., 2015; Vander Heiden et al., 2009). In this protocol, we describe a method for the determination of oxygen consumption rates in the nematode Caenorhabditis elegans.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
|---|---|
| Enthalten in: |
Bio-protocol - 6(2016), 23 vom: 05. Dez. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Palikaras, Konstantinos [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Ageing |
|---|
| Anmerkungen: |
Date Revised 10.02.2022 published: Print Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.21769/BioProtoc.2049 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM269315918 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM269315918 | ||
| 003 | DE-627 | ||
| 005 | 20231224224328.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.21769/BioProtoc.2049 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0897.xml |
| 035 | |a (DE-627)NLM269315918 | ||
| 035 | |a (NLM)28239622 | ||
| 035 | |a (PII)e2049 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Palikaras, Konstantinos |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Measuring Oxygen Consumption Rate in Caenorhabditis elegans |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 10.02.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a The rate of oxygen consumption is a vital marker indicating cellular function during lifetime under normal or metabolically challenged conditions. It is used broadly to study mitochondrial function (Artal-Sanz and Tavernarakis, 2009; Palikaras et al., 2015; Ryu et al., 2016) or investigate factors mediating the switch from oxidative phosphorylation to aerobic glycolysis (Chen et al., 2015; Vander Heiden et al., 2009). In this protocol, we describe a method for the determination of oxygen consumption rates in the nematode Caenorhabditis elegans | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Ageing | |
| 650 | 4 | |a Caenorhabditis elegans | |
| 650 | 4 | |a Metabolism | |
| 650 | 4 | |a Mitochondria | |
| 650 | 4 | |a Oxygen sensor | |
| 650 | 4 | |a ROS | |
| 700 | 1 | |a Tavernarakis, Nektarios |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Bio-protocol |d 2012 |g 6(2016), 23 vom: 05. Dez. |w (DE-627)NLM243846711 |x 2331-8325 |7 nnns |
| 773 | 1 | 8 | |g volume:6 |g year:2016 |g number:23 |g day:05 |g month:12 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.21769/BioProtoc.2049 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 6 |j 2016 |e 23 |b 05 |c 12 | ||