An In Vitro Assay for Sonothrombolysis Based on the Spectrophotometric Measurement of Clot Thickness
© 2017 by the American Institute of Ultrasound in Medicine..
OBJECTIVES: For improved thrombolysis therapy based on ultrasound irradiation, researchers and practitioners would strongly benefit from an easy and efficient in vitro assay system of thrombolysis activity involving irradiated ultrasound. For the present study, we designed a new in vitro sonothrombolysis assay system using a sheet-type clot.
METHODS: We designed a cell for clot assay, and we confirmed that this clot cell did not significantly intervene in the acoustic field. Using human plasma, we made a sheet-type clot in the cell. Clot thicknesses at 100 points along 4 directions were measured photometrically at a rate of approximately 4 points/s.
RESULTS: The sonothrombolysis effects at 13 levels of ultrasonic intensity were obtained with only one sheet-type clot. With this method, we used a clinically oriented probe at 0.7 and 0.3 W/cm2 to confirm that sonothrombolysis took place.
CONCLUSIONS: We successfully established a new, easy, and efficient method for conducting in vitro sonothrombolysis assays. This method involves little intervention of either ultrasound reflection or standing waves in the clot cell. We believe that this new assay method is very useful for fundamental analyses of ultrasound's thrombolysis effects.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
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| Enthalten in: |
Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine - 36(2017), 4 vom: 21. Apr., Seite 681-698 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Zuojun [VerfasserIn] |
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| Links: |
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| Themen: |
Acute ischemic stroke |
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| Anmerkungen: |
Date Completed 04.10.2017 Date Revised 04.10.2017 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.7863/ultra.15.11018 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM268570507 |
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| 100 | 1 | |a Wang, Zuojun |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An In Vitro Assay for Sonothrombolysis Based on the Spectrophotometric Measurement of Clot Thickness |
| 264 | 1 | |c 2017 | |
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| 500 | |a Date Completed 04.10.2017 | ||
| 500 | |a Date Revised 04.10.2017 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2017 by the American Institute of Ultrasound in Medicine. | ||
| 520 | |a OBJECTIVES: For improved thrombolysis therapy based on ultrasound irradiation, researchers and practitioners would strongly benefit from an easy and efficient in vitro assay system of thrombolysis activity involving irradiated ultrasound. For the present study, we designed a new in vitro sonothrombolysis assay system using a sheet-type clot | ||
| 520 | |a METHODS: We designed a cell for clot assay, and we confirmed that this clot cell did not significantly intervene in the acoustic field. Using human plasma, we made a sheet-type clot in the cell. Clot thicknesses at 100 points along 4 directions were measured photometrically at a rate of approximately 4 points/s | ||
| 520 | |a RESULTS: The sonothrombolysis effects at 13 levels of ultrasonic intensity were obtained with only one sheet-type clot. With this method, we used a clinically oriented probe at 0.7 and 0.3 W/cm2 to confirm that sonothrombolysis took place | ||
| 520 | |a CONCLUSIONS: We successfully established a new, easy, and efficient method for conducting in vitro sonothrombolysis assays. This method involves little intervention of either ultrasound reflection or standing waves in the clot cell. We believe that this new assay method is very useful for fundamental analyses of ultrasound's thrombolysis effects | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a acute ischemic stroke | |
| 650 | 4 | |a fibrin clot | |
| 650 | 4 | |a rt-PA | |
| 650 | 4 | |a sonothrombolysis | |
| 650 | 4 | |a standing wave | |
| 700 | 1 | |a Sawaguchi, Yoshikazu |e verfasserin |4 aut | |
| 700 | 1 | |a Hirose, Hideo |e verfasserin |4 aut | |
| 700 | 1 | |a Ohara, Kazumasa |e verfasserin |4 aut | |
| 700 | 1 | |a Sakamoto, Shinichiro |e verfasserin |4 aut | |
| 700 | 1 | |a Mitsumura, Hidetaka |e verfasserin |4 aut | |
| 700 | 1 | |a Ogawa, Takeki |e verfasserin |4 aut | |
| 700 | 1 | |a Iguchi, Yasuyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Yokoyama, Masayuki |e verfasserin |4 aut | |
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