Electronic cigarettes increase endothelial progenitor cells in the blood of healthy volunteers
Copyright © 2016 Elsevier Ireland Ltd. All rights reserved..
BACKGROUND AND AIMS: The use of electronic cigarettes is increasing dramatically on a global scale and its effects on human health remain uncertain. In the present study, we measured endothelial progenitor cells (EPCs) and microvesicles (MVs) in healthy young volunteers following short-term exposure to inhalation of e-cigarette vapor (ECV) to determine vascular changes.
METHODS: Sixteen healthy seldom smokers were randomized into two groups either exposed or not exposed to 10 puffs of ECV for 10 min, in a crossover design. Blood samples were obtained at baseline and 1, 4 and 24 h following exposure. EPCs (CD34 + CD309) and MVs were analyzed by flow cytometry. MVs were phenotyped according to origin (platelet (CD41), endothelial (CD144), leukocytes (CD45), monocytes (CD14)) and nuclear content (SYTO 13 dye). In addition, expression of inflammation markers such P-selectin (CD62P), E-selectin (CD62E), CD40-ligand (CD154) and HMGB1 was investigated. Fractional exhaled nitric oxide (FeNO) was also measured at baseline and after 24 h.
RESULTS: EPC levels in blood were significantly increased 1 h following exposure to ECV and returned to baseline values after 24 h. Only E-selectin positive MVs (endothelial origin) were slightly elevated (p < 0.038). FeNO was unaffected by exposure to ECV.
CONCLUSIONS: In healthy volunteers, ten puffs of e-cigarette vapor inhalation caused an increase in EPCs. This increase was of the same magnitude as following smoking of one traditional cigarette, as we previously demonstrated. Taken together, these results may represent signs of possible vascular changes after short e-cigarette inhalation. Further studies analyzing potential cardiovascular health effects are critical as the e-cigarette market continues to burgeon.
| Errataetall: |
CommentIn: Atherosclerosis. 2016 Dec;255:119-121. - PMID 27814909 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:255 |
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| Enthalten in: |
Atherosclerosis - 255(2016) vom: 01. Dez., Seite 179-185 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Antoniewicz, Lukasz [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 06.11.2017 Date Revised 18.05.2018 published: Print-Electronic CommentIn: Atherosclerosis. 2016 Dec;255:119-121. - PMID 27814909 Citation Status MEDLINE |
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| doi: |
10.1016/j.atherosclerosis.2016.09.064 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM264899571 |
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| 100 | 1 | |a Antoniewicz, Lukasz |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Electronic cigarettes increase endothelial progenitor cells in the blood of healthy volunteers |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a CommentIn: Atherosclerosis. 2016 Dec;255:119-121. - PMID 27814909 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier Ireland Ltd. All rights reserved. | ||
| 520 | |a BACKGROUND AND AIMS: The use of electronic cigarettes is increasing dramatically on a global scale and its effects on human health remain uncertain. In the present study, we measured endothelial progenitor cells (EPCs) and microvesicles (MVs) in healthy young volunteers following short-term exposure to inhalation of e-cigarette vapor (ECV) to determine vascular changes | ||
| 520 | |a METHODS: Sixteen healthy seldom smokers were randomized into two groups either exposed or not exposed to 10 puffs of ECV for 10 min, in a crossover design. Blood samples were obtained at baseline and 1, 4 and 24 h following exposure. EPCs (CD34 + CD309) and MVs were analyzed by flow cytometry. MVs were phenotyped according to origin (platelet (CD41), endothelial (CD144), leukocytes (CD45), monocytes (CD14)) and nuclear content (SYTO 13 dye). In addition, expression of inflammation markers such P-selectin (CD62P), E-selectin (CD62E), CD40-ligand (CD154) and HMGB1 was investigated. Fractional exhaled nitric oxide (FeNO) was also measured at baseline and after 24 h | ||
| 520 | |a RESULTS: EPC levels in blood were significantly increased 1 h following exposure to ECV and returned to baseline values after 24 h. Only E-selectin positive MVs (endothelial origin) were slightly elevated (p < 0.038). FeNO was unaffected by exposure to ECV | ||
| 520 | |a CONCLUSIONS: In healthy volunteers, ten puffs of e-cigarette vapor inhalation caused an increase in EPCs. This increase was of the same magnitude as following smoking of one traditional cigarette, as we previously demonstrated. Taken together, these results may represent signs of possible vascular changes after short e-cigarette inhalation. Further studies analyzing potential cardiovascular health effects are critical as the e-cigarette market continues to burgeon | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Randomized Controlled Trial | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a E-cigarette | |
| 650 | 4 | |a Electronic cigarette | |
| 650 | 4 | |a Endothelial progenitor cells | |
| 650 | 4 | |a Microvesicles | |
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| 650 | 7 | |a Inflammation Mediators |2 NLM | |
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| 700 | 1 | |a Abdel-Halim, Samy M |e verfasserin |4 aut | |
| 700 | 1 | |a Kiessling, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Mobarrez, Fariborz |e verfasserin |4 aut | |
| 700 | 1 | |a Lundbäck, Magnus |e verfasserin |4 aut | |
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