Identification of growing bacteria during litter decomposition in freshwater through H218O quantitative stable isotope probing
© 2016 Society for Applied Microbiology and John Wiley & Sons Ltd..
Identification of microorganisms that facilitate the cycling of nutrients in freshwater is paramount to understanding how these ecosystems function. Here, we identify growing aquatic bacteria using H218O quantitative stable isotope probing. During 8 day incubations in 97 atom % H218O, 54% of the taxa grew. The most abundant phyla among growing taxa were Proteobacteria (45%), Bacteroidetes (30%) and Firmicutes (10%). Taxa differed in isotopic enrichment, reflecting variation in DNA replication of bacterial populations. At the class level, the highest atom fraction excess was observed for OPB41 and δ-Proteobacteria. There was no linear relationship between 18 O incorporation and abundance of taxa. δ-Proteobacteria and OPB41 were not abundant, yet the DNA of both taxa was highly enriched in 18 O. Bacteriodetes, in contrast, were abundant but not highly enriched. Our study shows that a large proportion of the bacterial taxa found on decomposing leaf litter grew slowly, and several low abundance taxa were highly enriched. These findings indicating that rare organisms may be important for the decomposition of leaf litter in streams, and that quantitative stable isotope probing with H218O can be used to advance our understanding of microorganisms in freshwater by identifying species that are growing in complex communities.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2016 |
|---|---|
| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
|---|---|
| Enthalten in: |
Environmental microbiology reports - 8(2016), 6 vom: 02. Dez., Seite 975-982 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Hayer, Michaela [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Completed 29.08.2017 Date Revised 26.11.2017 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/1758-2229.12475 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM264589165 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM264589165 | ||
| 003 | DE-627 | ||
| 005 | 20231224210427.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/1758-2229.12475 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0882.xml |
| 035 | |a (DE-627)NLM264589165 | ||
| 035 | |a (NLM)27657357 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Hayer, Michaela |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification of growing bacteria during litter decomposition in freshwater through H218O quantitative stable isotope probing |
| 264 | 1 | |c 2016 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 29.08.2017 | ||
| 500 | |a Date Revised 26.11.2017 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd. | ||
| 520 | |a Identification of microorganisms that facilitate the cycling of nutrients in freshwater is paramount to understanding how these ecosystems function. Here, we identify growing aquatic bacteria using H218O quantitative stable isotope probing. During 8 day incubations in 97 atom % H218O, 54% of the taxa grew. The most abundant phyla among growing taxa were Proteobacteria (45%), Bacteroidetes (30%) and Firmicutes (10%). Taxa differed in isotopic enrichment, reflecting variation in DNA replication of bacterial populations. At the class level, the highest atom fraction excess was observed for OPB41 and δ-Proteobacteria. There was no linear relationship between 18 O incorporation and abundance of taxa. δ-Proteobacteria and OPB41 were not abundant, yet the DNA of both taxa was highly enriched in 18 O. Bacteriodetes, in contrast, were abundant but not highly enriched. Our study shows that a large proportion of the bacterial taxa found on decomposing leaf litter grew slowly, and several low abundance taxa were highly enriched. These findings indicating that rare organisms may be important for the decomposition of leaf litter in streams, and that quantitative stable isotope probing with H218O can be used to advance our understanding of microorganisms in freshwater by identifying species that are growing in complex communities | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 700 | 1 | |a Schwartz, Egbert |e verfasserin |4 aut | |
| 700 | 1 | |a Marks, Jane C |e verfasserin |4 aut | |
| 700 | 1 | |a Koch, Benjamin J |e verfasserin |4 aut | |
| 700 | 1 | |a Morrissey, Ember M |e verfasserin |4 aut | |
| 700 | 1 | |a Schuettenberg, Alexa A |e verfasserin |4 aut | |
| 700 | 1 | |a Hungate, Bruce A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Environmental microbiology reports |d 2009 |g 8(2016), 6 vom: 02. Dez., Seite 975-982 |w (DE-627)NLM192135570 |x 1758-2229 |7 nnns |
| 773 | 1 | 8 | |g volume:8 |g year:2016 |g number:6 |g day:02 |g month:12 |g pages:975-982 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/1758-2229.12475 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 8 |j 2016 |e 6 |b 02 |c 12 |h 975-982 | ||