An optimized, highly efficient, self-assembled, subvirus-like particle of infectious bursal disease virus (IBDV)
Copyright © 2016. Published by Elsevier Ltd..
Infectious bursal disease virus (IBDV) causes immunosuppression in young chickens, leading to increased susceptibility to other diseases and a reduction in the immune response to other vaccines. Thus, IBDV results in great economic losses to the poultry industry. The most effective method of prevention is vaccination. However, medium-virulence vaccines can cause bursal pathological damage and immunosuppression. Here, we describe a safer, self-assembled, subvirus-like particle (sVP) vaccine without a complex purification process. The IBD-VP2 gene was cloned into Pichia pastoris, and the expressed protein self-assembled into T=1 sVPs (∼23nm). Immunization experiments showed that the sVP vaccine elicited high IBDV-neutralizing antibodies in each group, and all birds survived challenge with very virulent IBDV (vvIBDV). Additionally, IBDV RNA was not detected, and sterile immunity was achieved. In conclusion, the IBD-sVP is a suitable candidate for a recombinant subunit vaccine against IBDV.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
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| Enthalten in: |
Vaccine - 34(2016), 30 vom: 24. Juni, Seite 3508-14 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Miao [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 26.10.2017 Date Revised 04.12.2017 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.vaccine.2016.02.072 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM260208582 |
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| 100 | 1 | |a Wang, Miao |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An optimized, highly efficient, self-assembled, subvirus-like particle of infectious bursal disease virus (IBDV) |
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| 500 | |a Date Completed 26.10.2017 | ||
| 500 | |a Date Revised 04.12.2017 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016. Published by Elsevier Ltd. | ||
| 520 | |a Infectious bursal disease virus (IBDV) causes immunosuppression in young chickens, leading to increased susceptibility to other diseases and a reduction in the immune response to other vaccines. Thus, IBDV results in great economic losses to the poultry industry. The most effective method of prevention is vaccination. However, medium-virulence vaccines can cause bursal pathological damage and immunosuppression. Here, we describe a safer, self-assembled, subvirus-like particle (sVP) vaccine without a complex purification process. The IBD-VP2 gene was cloned into Pichia pastoris, and the expressed protein self-assembled into T=1 sVPs (∼23nm). Immunization experiments showed that the sVP vaccine elicited high IBDV-neutralizing antibodies in each group, and all birds survived challenge with very virulent IBDV (vvIBDV). Additionally, IBDV RNA was not detected, and sterile immunity was achieved. In conclusion, the IBD-sVP is a suitable candidate for a recombinant subunit vaccine against IBDV | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Infectious bursal disease virus | |
| 650 | 4 | |a Yeast | |
| 650 | 4 | |a sVP | |
| 650 | 7 | |a Antibodies, Neutralizing |2 NLM | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a VP2 protein, infectious bursal disease virus |2 NLM | |
| 650 | 7 | |a Vaccines, Subunit |2 NLM | |
| 650 | 7 | |a Vaccines, Synthetic |2 NLM | |
| 650 | 7 | |a Vaccines, Virus-Like Particle |2 NLM | |
| 650 | 7 | |a Viral Structural Proteins |2 NLM | |
| 650 | 7 | |a Viral Vaccines |2 NLM | |
| 700 | 1 | |a Pan, Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Zhen |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Honglei |e verfasserin |4 aut | |
| 700 | 1 | |a Qi, Xiaole |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Yulong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaomei |e verfasserin |4 aut | |
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