Highly Efficient Synthesis of an Emerging Lipophilic Antioxidant : 2-Ethylhexyl Ferulate
Ferulic acid in ester form has shown a stronger ability in ameliorating certain pathological conditions and inhibiting lipid oxidation. In present study, a solvent-free and reduced pressure evaporation system was developed for lipase-catalyzed synthesis of 2-ethylhexyl ferulate (2-EF) from ferulic acid and 2-ethylhexanol. A Box-Behnken design with response surface methodology (RSM) and artificial neural network (ANN) was selected to model and optimize the process. Based on the yields of 2-EF, reaction temperature was shown to be the most important process factor on the molar conversion among all variables. The residual values and the coefficient of determination (R²) calculated from the design data indicated that ANN was better than RSM in data fitting. Overall, the present lipase-catalyzed approach for 2-EF synthesis at low reaction temperature in a reduced pressure evaporation system shows high 2-EF production efficiency. Notably, this approach can reduce the enzyme denaturation and ferulic acid oxidation that usually occur during long-term biosynthetic operations at high temperature.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
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| Enthalten in: |
Molecules (Basel, Switzerland) - 21(2016), 4 vom: 12. Apr., Seite 478 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Huang, Kuo-Chuan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 26.12.2016 Date Revised 25.02.2020 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/molecules21040478 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM259397466 |
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| 500 | |a Date Revised 25.02.2020 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Ferulic acid in ester form has shown a stronger ability in ameliorating certain pathological conditions and inhibiting lipid oxidation. In present study, a solvent-free and reduced pressure evaporation system was developed for lipase-catalyzed synthesis of 2-ethylhexyl ferulate (2-EF) from ferulic acid and 2-ethylhexanol. A Box-Behnken design with response surface methodology (RSM) and artificial neural network (ANN) was selected to model and optimize the process. Based on the yields of 2-EF, reaction temperature was shown to be the most important process factor on the molar conversion among all variables. The residual values and the coefficient of determination (R²) calculated from the design data indicated that ANN was better than RSM in data fitting. Overall, the present lipase-catalyzed approach for 2-EF synthesis at low reaction temperature in a reduced pressure evaporation system shows high 2-EF production efficiency. Notably, this approach can reduce the enzyme denaturation and ferulic acid oxidation that usually occur during long-term biosynthetic operations at high temperature | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a 2-ethylhexyl ferulate | |
| 650 | 4 | |a artificial neural network | |
| 650 | 4 | |a lipase | |
| 650 | 4 | |a reduced pressure evaporation system | |
| 650 | 4 | |a response surface methodology | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Coumaric Acids |2 NLM | |
| 650 | 7 | |a Esters |2 NLM | |
| 650 | 7 | |a Hexanols |2 NLM | |
| 650 | 7 | |a Solvents |2 NLM | |
| 650 | 7 | |a ferulic acid |2 NLM | |
| 650 | 7 | |a AVM951ZWST |2 NLM | |
| 650 | 7 | |a Lipase |2 NLM | |
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| 700 | 1 | |a Li, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Kuo, Chia-Hung |e verfasserin |4 aut | |
| 700 | 1 | |a Twu, Yawo-Kuo |e verfasserin |4 aut | |
| 700 | 1 | |a Shieh, Chwen-Jen |e verfasserin |4 aut | |
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