Transient Receptor Potential Melastatin-3 (TRPM3) Mediates Nociceptive-Like Responses in Hydra vulgaris

The ability of mammals to feel noxious stimuli lies in a heterogeneous group of primary somatosensory neurons termed nociceptors, which express specific membrane receptors, such as the Transient Receptor Potential (TRP) family. Here, we show that one of the most important nociceptive-like pathways is conserved in the freshwater coelenterate Hydra vulgaris, the most primitive organism possessing a nervous system. In particular, we found that H. vulgaris expresses TRPM3, a nociceptor calcium channel involved in the detection of noxious heat in mammals. Furthermore, we detected that both heat shock and TRPM3 specific agonist (i.e., pregnenolone sulfate) induce the modulation of the heat shock protein 70 (HSP70) and the nitric oxide synthase (NOS), two genes activated by TRP-mediated heat painful stimuli in mammals. As expected, these effects are inhibited by a TRPM3 antagonist (i.e., mefenamic acid). Interestingly, the TRPM3 agonist and heat shock also induce the expression of nuclear transcription erythroid 2-related factor (Nrf2) and superoxide dismutase (SOD), known markers of oxidative stress; noteworthy gene expression was also inhibited by the TRPM3 antagonist. As a whole, our results demonstrate the presence of conserved molecular oxidative/nociceptive-like pathways at the primordial level of the animal kingdom.

Medienart:

E-Artikel

Erscheinungsjahr:

2016

Erschienen:

2016

Enthalten in:

Zur Gesamtaufnahme - volume:11

Enthalten in:

PloS one - 11(2016), 3 vom: 15., Seite e0151386

Sprache:

Englisch

Beteiligte Personen:

Malafoglia, Valentina [VerfasserIn]
Traversetti, Lorenzo [VerfasserIn]
Del Grosso, Floriano [VerfasserIn]
Scalici, Massimiliano [VerfasserIn]
Lauro, Filomena [VerfasserIn]
Russo, Valeria [VerfasserIn]
Persichini, Tiziana [VerfasserIn]
Salvemini, Daniela [VerfasserIn]
Mollace, Vincenzo [VerfasserIn]
Fini, Massimo [VerfasserIn]
Raffaeli, William [VerfasserIn]
Muscoli, Carolina [VerfasserIn]
Colasanti, Marco [VerfasserIn]

Links:

Volltext

Themen:

04Y4D91RG0
73R90F7MQ8
EC 1.15.1.1
HSP70 Heat-Shock Proteins
Journal Article
Pregnenolone
Pregnenolone sulfate
RNA, Messenger
Research Support, Non-U.S. Gov't
Superoxide Dismutase
TRPM Cation Channels

Anmerkungen:

Date Completed 08.08.2016

Date Revised 11.11.2023

published: Electronic-eCollection

Citation Status MEDLINE

doi:

10.1371/journal.pone.0151386

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM258396865