Robust neuroprotective effects of intranasally delivered iNOS siRNA encapsulated in gelatin nanoparticles in the postischemic brain
Copyright © 2016 Elsevier Inc. All rights reserved..
The therapeutic efficacy of intranasal iNOS siRNA delivery was investigated in the postischemic rat brain after encapsulating on in gelatin nanoparticles (GNPs; diameter 188.0 ± 60.9 nm) cross-linked with 0.0667% glutaraldehyde (GA). Intranasally delivered GNPs were found in extracellular and intracellular compartments of many brain regions, including the olfactory bulb, cerebral cortex, and striatum at 1 hour after infusion and continued to be detected for days. Infarct volumes were markedly suppressed (maximal reduction to 42.1 ± 2.6%) at 2 days after 60 minutes of middle cerebral artery occlusion (MCAO) when iNOS siRNA/GNPs were delivered at 6 hours post-MCAO. In addition, this protective effect was manifested by reductions in neurological and behavioral deficits that were sustained for 2 weeks. Therapeutic potency of iNOS siRNA/GNPs was significantly greater and sustained longer than that of bare siRNA and prolonged and efficient iNOS by iNOS siRNA/GNP is responsible for the robust neuroprotective effect.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2016 |
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| Erschienen: |
2016 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Nanomedicine : nanotechnology, biology, and medicine - 12(2016), 5 vom: 01. Juli, Seite 1219-29 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kim, Il-Doo [VerfasserIn] |
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| Links: |
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| Themen: |
9000-70-8 |
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| Anmerkungen: |
Date Completed 02.10.2017 Date Revised 02.12.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.nano.2016.01.002 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM258132221 |
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| 245 | 1 | 0 | |a Robust neuroprotective effects of intranasally delivered iNOS siRNA encapsulated in gelatin nanoparticles in the postischemic brain |
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| 500 | |a Date Revised 02.12.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2016 Elsevier Inc. All rights reserved. | ||
| 520 | |a The therapeutic efficacy of intranasal iNOS siRNA delivery was investigated in the postischemic rat brain after encapsulating on in gelatin nanoparticles (GNPs; diameter 188.0 ± 60.9 nm) cross-linked with 0.0667% glutaraldehyde (GA). Intranasally delivered GNPs were found in extracellular and intracellular compartments of many brain regions, including the olfactory bulb, cerebral cortex, and striatum at 1 hour after infusion and continued to be detected for days. Infarct volumes were markedly suppressed (maximal reduction to 42.1 ± 2.6%) at 2 days after 60 minutes of middle cerebral artery occlusion (MCAO) when iNOS siRNA/GNPs were delivered at 6 hours post-MCAO. In addition, this protective effect was manifested by reductions in neurological and behavioral deficits that were sustained for 2 weeks. Therapeutic potency of iNOS siRNA/GNPs was significantly greater and sustained longer than that of bare siRNA and prolonged and efficient iNOS by iNOS siRNA/GNP is responsible for the robust neuroprotective effect | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Gelatin nanoparticles | |
| 650 | 4 | |a Intranasal delivery | |
| 650 | 4 | |a MCAO | |
| 650 | 4 | |a iNOS | |
| 650 | 4 | |a siRNA | |
| 650 | 7 | |a Neuroprotective Agents |2 NLM | |
| 650 | 7 | |a RNA, Small Interfering |2 NLM | |
| 650 | 7 | |a Gelatin |2 NLM | |
| 650 | 7 | |a 9000-70-8 |2 NLM | |
| 700 | 1 | |a Sawicki, Elizabeth |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Hye-Kyung |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Eun-Hwa |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Heon Joo |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Pyung-Lim |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Kyekyoon Kevin |e verfasserin |4 aut | |
| 700 | 1 | |a Choi, Hyungsoo |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Ja-Kyeong |e verfasserin |4 aut | |
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