In vitro and in vivo evaluation of calcium phosphate composite scaffolds containing BMP-VEGF loaded PLGA microspheres for the treatment of avascular necrosis of the femoral head

Copyright © 2015 Elsevier B.V. All rights reserved..

Avascular necrosis of the femoral head (ANFH) is difficult to treat due to high pressure and hypoxia, and reduced levels of growth factors such as bone morphogenetic protein (BMP), and vascular endothelial growth factor (VEGF). We generated a novel calcium phosphate (CPC) composite scaffold, which contains BMP-VEGF-loaded poly-lactic-co-glycolic acid (PLGA) microspheres (BMP-VEGF-PLGA-CPC). The BMP-VEGF-loaded microspheres have an encapsulation efficiency of 89.15% for BMP, and 78.55% for VEGF. The BMP-VEGF-PLGA-CPC scaffold also demonstrated a porosity of 62% with interconnected porous structures, and pore sizes of 219 μm and compressive strength of 6.60 MPa. Additionally, bone marrow mesenchymal stem cells (BMSCs) were seeded on scaffolds in vitro. Further characterization showed that the BMP-VEGF-PLGA-CPC scaffolds were biocompatible and enhanced osteogenesis and angiogenesis in vitro. Using a rabbit model of ANFH, BMP-VEGF-PLGA-CPC scaffolds were implanted into the bone tunnels of core decompression in the femoral head for 6 and 12 weeks. Radiographic and histological analysis demonstrated that the BMP-VEGF-PLGA-CPC scaffolds exhibited good biocompatibility, and osteogenic and angiogenic activity in vivo. These results indicate that the BMP-VEGF-PLGA-CPC scaffold may improve the therapeutic effect of core decompression surgery and be used as a treatment for ANFH.

Medienart:

E-Artikel

Erscheinungsjahr:

2016

Erschienen:

2016

Enthalten in:

Zur Gesamtaufnahme - volume:60

Enthalten in:

Materials science & engineering. C, Materials for biological applications - 60(2016) vom: 15. März, Seite 298-307

Sprache:

Englisch

Beteiligte Personen:

Zhang, Hao-Xuan [VerfasserIn]
Zhang, Xiu-Ping [VerfasserIn]
Xiao, Gui-Yong [VerfasserIn]
Hou, Yong [VerfasserIn]
Cheng, Lei [VerfasserIn]
Si, Meng [VerfasserIn]
Wang, Shuai-Shuai [VerfasserIn]
Li, Yu-Hua [VerfasserIn]
Nie, Lin [VerfasserIn]

Links:

Volltext

Themen:

1SIA8062RS
26009-03-0
33X04XA5AT
Avascular necrosis of the femoral head
Biocompatible Materials
Bone Morphogenetic Proteins
Bone tissue engineering
Core decompression
Journal Article
Lactic Acid
Polyglycolic Acid
Polylactic Acid-Polyglycolic Acid Copolymer
Research Support, Non-U.S. Gov't
Scaffolds
Vascular Endothelial Growth Factor A

Anmerkungen:

Date Completed 26.09.2016

Date Revised 31.03.2022

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.msec.2015.11.055

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM255920180