A Novel Natural Product, KL-21, Inhibits Proliferation and Induces Apoptosis in Chronic Lymphocytic Leukemia Cells

OBJECTIVE: The aims of this study were to examine the cytotoxic and apoptotic effects of KL-21, a novel plant product (produced by Naturin Natural Products, İzmir, Turkey), on 232B4 chronic lymphocytic leukemia (CLL) cells and to determine the cytotoxic effects on healthy BEAS-2B human bronchial epithelial cells.

MATERIALS AND METHODS: The cytotoxic effect of KL-21 was determined by MTT cell proliferation assay. Changes in caspase-3 enzyme activity were measured using the caspase-3 colorimetric assay. Changes in mitochondrial membrane potential were determined using the JC-1 dye-based method. Annexin V-FITC/PI double staining was performed to measure the apoptotic cell population. Effects of KL-21 on cell cycle profiles of CLL cells were investigated by flow cytometry.

RESULTS: We detected time- and concentration-dependent increases in the cytotoxic effect of KL-21 on 232B4 CLL cells. However, we also showed that, especially at higher concentrations, KL-21 was less cytotoxic towards BEAS-2B healthy cells than towards CLL cells. Annexin-V/PI double staining results showed that the apoptotic cell population increased in 232B4 cells. Increasing concentrations of KL-21 increased caspase-3 enzyme activity and induced loss of mitochondrial membrane potential. KL-21 administration resulted in small increases in the percentage of the cells in the G0/G1 phase while it decreased the S phase cell population up to 1 mg/mL. At the highest concentration, most of the cells accumulated in the G0/G1 phase.

CONCLUSION: KL-21 has a growth-inhibitory effect on 232B4 CLL cells. KL-21 causes apoptosis and cell cycle arrest at G0/G1.

Medienart:

E-Artikel

Erscheinungsjahr:

2015

Erschienen:

2015

Enthalten in:

Zur Gesamtaufnahme - volume:32

Enthalten in:

Turkish journal of haematology : official journal of Turkish Society of Haematology - 32(2015), 2 vom: 05. Juni, Seite 118-26

Sprache:

Englisch

Beteiligte Personen:

Adan Gökbulut, Aysun [VerfasserIn]
Yaşar, Mustafa [VerfasserIn]
Baran, Yusuf [VerfasserIn]

Links:

Volltext

Themen:

Antineoplastic Agents, Phytogenic
Biological Products
CASP3 protein, human
Caspase 3
EC 3.4.22.-
Journal Article
KL-21 plant preparation
Neoplasm Proteins
Plant Extracts

Anmerkungen:

Date Completed 23.01.2017

Date Revised 13.11.2018

published: Print

Citation Status MEDLINE

doi:

10.4274/tjh.2013.0381

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM252248902