Spatial, Temporal, and Matrix Variability of Clostridium botulinum Type E Toxin Gene Distribution at Great Lakes Beaches

Copyright © 2015, American Society for Microbiology. All Rights Reserved..

Clostridium botulinum type E toxin is responsible for extensive mortality of birds and fish in the Great Lakes. The C. botulinum bontE gene that produces the type E toxin was amplified with quantitative PCR from 150 sloughed algal samples (primarily Cladophora species) collected during summer 2012 from 10 Great Lakes beaches in five states; concurrently, 74 sediment and 37 water samples from four sites were also analyzed. The bontE gene concentration in algae was significantly higher than in water and sediment (P < 0.05), suggesting that algal mats provide a better microenvironment for C. botulinum. The bontE gene was detected most frequently in algae at Jeorse Park and Portage Lake Front beaches (Lake Michigan) and Bay City State Recreation Area beach on Saginaw Bay (Lake Huron), where 77, 100, and 83% of these algal samples contained the bontE gene, respectively. The highest concentration of bontE was detected at Bay City (1.98 × 10(5) gene copies/ml of algae or 5.21 × 10(6) g [dry weight]). This study revealed that the bontE gene is abundant in the Great Lakes but that it has spatial, temporal, and matrix variability. Further, embayed beaches, low wave height, low wind velocity, and greater average water temperature enhance the bontE occurrence.

Medienart:

E-Artikel

Erscheinungsjahr:

2015

Erschienen:

2015

Enthalten in:

Zur Gesamtaufnahme - volume:81

Enthalten in:

Applied and environmental microbiology - 81(2015), 13 vom: 12. Juli, Seite 4306-15

Sprache:

Englisch

Beteiligte Personen:

Wijesinghe, Rasanthi U [VerfasserIn]
Oster, Ryan J [VerfasserIn]
Haack, Sheridan K [VerfasserIn]
Fogarty, Lisa R [VerfasserIn]
Tucker, Taaja R [VerfasserIn]
Riley, Stephen C [VerfasserIn]

Links:

Volltext

Themen:

DNA, Bacterial
Journal Article
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 25.02.2016

Date Revised 13.11.2018

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1128/AEM.00098-15

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM248191063