Detection of Schistosoma mansoni infection by TaqMan® Real-Time PCR in a hamster model
Copyright © 2014 Elsevier Inc. All rights reserved..
An experimental study in hamsters was performed to evaluate the capability for detecting Schistosoma mansoni DNA in serum and fecal samples during the pre and post-egg-laying periods of infection using TaqMan® Real-Time PCR system (qPCR), was compared with the circumoval precipitin test (COPT) and the Kato-Katz technique, especially among individuals with low parasitic burden. Twenty-four hamsters were infected with cercariae. Three hamsters were sacrificed per week under anesthesia, from 7 days post infection (DPI) up to 56 DPI. A serum sample and a pool of feces were collected from each hamster. The presence of S. mansoni eggs in fecal samples was evaluated by Kato-Katz method and in the hamsters gutby histopathology. Detection of S. mansoni DNA was performed using qPCR and S. mansoni antibody using COPT. The first detection of eggs in feces by Kato-Katz method and S. mansoni DNA in feces by qPCR occurred 49 DPI. Nevertheless, S. mansoni DNA was detected in serum samples from 14 up to 56 DPI. COPT was positive at 35 DPI. The results not only confirm the reliability of S. mansoni DNA detection by qPCR, but also demonstrate that serum is a trustworthy source of DNA in the pre patent infection period.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2014 |
|---|---|
| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:143 |
|---|---|
| Enthalten in: |
Experimental parasitology - 143(2014) vom: 01. Aug., Seite 83-9 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Espírito-Santo, Maria Cristina Carvalho [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Circumoval precipitin test |
|---|
| Anmerkungen: |
Date Completed 08.09.2014 Date Revised 21.07.2014 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.exppara.2014.05.013 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM238536394 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM238536394 | ||
| 003 | DE-627 | ||
| 005 | 20231224114014.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2014 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.exppara.2014.05.013 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0795.xml |
| 035 | |a (DE-627)NLM238536394 | ||
| 035 | |a (NLM)24858959 | ||
| 035 | |a (PII)S0014-4894(14)00127-1 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Espírito-Santo, Maria Cristina Carvalho |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detection of Schistosoma mansoni infection by TaqMan® Real-Time PCR in a hamster model |
| 264 | 1 | |c 2014 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 08.09.2014 | ||
| 500 | |a Date Revised 21.07.2014 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2014 Elsevier Inc. All rights reserved. | ||
| 520 | |a An experimental study in hamsters was performed to evaluate the capability for detecting Schistosoma mansoni DNA in serum and fecal samples during the pre and post-egg-laying periods of infection using TaqMan® Real-Time PCR system (qPCR), was compared with the circumoval precipitin test (COPT) and the Kato-Katz technique, especially among individuals with low parasitic burden. Twenty-four hamsters were infected with cercariae. Three hamsters were sacrificed per week under anesthesia, from 7 days post infection (DPI) up to 56 DPI. A serum sample and a pool of feces were collected from each hamster. The presence of S. mansoni eggs in fecal samples was evaluated by Kato-Katz method and in the hamsters gutby histopathology. Detection of S. mansoni DNA was performed using qPCR and S. mansoni antibody using COPT. The first detection of eggs in feces by Kato-Katz method and S. mansoni DNA in feces by qPCR occurred 49 DPI. Nevertheless, S. mansoni DNA was detected in serum samples from 14 up to 56 DPI. COPT was positive at 35 DPI. The results not only confirm the reliability of S. mansoni DNA detection by qPCR, but also demonstrate that serum is a trustworthy source of DNA in the pre patent infection period | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Circumoval precipitin test | |
| 650 | 4 | |a Histopathology | |
| 650 | 4 | |a Kato-Katz method | |
| 650 | 4 | |a S. mansoni | |
| 650 | 4 | |a TaqMan® Real-Time PCR system | |
| 650 | 7 | |a DNA, Helminth |2 NLM | |
| 700 | 1 | |a Alvarado-Mora, Mónica Viviana |e verfasserin |4 aut | |
| 700 | 1 | |a Pinto, Pedro Luiz Silva |e verfasserin |4 aut | |
| 700 | 1 | |a de Brito, Thales |e verfasserin |4 aut | |
| 700 | 1 | |a Botelho-Lima, Lívia |e verfasserin |4 aut | |
| 700 | 1 | |a Heath, Ashley Richard |e verfasserin |4 aut | |
| 700 | 1 | |a Amorim, Maria Galli |e verfasserin |4 aut | |
| 700 | 1 | |a Dias-Neto, Emmanuel |e verfasserin |4 aut | |
| 700 | 1 | |a Chieffi, Pedro Paulo |e verfasserin |4 aut | |
| 700 | 1 | |a Pinho, João Renato Rebello |e verfasserin |4 aut | |
| 700 | 1 | |a Carrilho, Flair José |e verfasserin |4 aut | |
| 700 | 1 | |a Luna, Expedito José Albuquerque |e verfasserin |4 aut | |
| 700 | 1 | |a Gryschek, Ronaldo Cesar Borges |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Experimental parasitology |d 1953 |g 143(2014) vom: 01. Aug., Seite 83-9 |w (DE-627)NLM000040436 |x 1090-2449 |7 nnns |
| 773 | 1 | 8 | |g volume:143 |g year:2014 |g day:01 |g month:08 |g pages:83-9 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.exppara.2014.05.013 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 143 |j 2014 |b 01 |c 08 |h 83-9 | ||