No effect of the transforming growth factor β1 promoter polymorphism C-509T on TGFB1 gene expression, protein secretion, or cellular radiosensitivity
Copyright © 2013 Elsevier Inc. All rights reserved..
PURPOSE: To study whether the promoter polymorphism (C-509T) affects transforming growth factor β1 gene (TGFB1) expression, protein secretion, and/or cellular radiosensitivity for both human lymphocytes and fibroblasts.
METHODS AND MATERIALS: Experiments were performed with lymphocytes taken either from 124 breast cancer patients or 59 pairs of normal monozygotic twins. We used 15 normal human primary fibroblast strains as controls. The C-509T genotype was determined by polymerase chain reaction-restriction fragment length polymorphism or TaqMan single nucleotide polymorphism (SNP) genotyping assay. The cellular radiosensitivity of lymphocytes was measured by G0/1 assay and that of fibroblasts by colony assay. The amount of extracellular TGFB1 protein was determined by enzyme-linked immunosorbent assay, and TGFB1 expression was assessed via microarray analysis or reverse transcription-polymerase chain reaction.
RESULTS: The C-509T genotype was found not to be associated with cellular radiosensitivity, neither for lymphocytes (breast cancer patients, P=.811; healthy donors, P=.181) nor for fibroblasts (P=.589). Both TGFB1 expression and TGFB1 protein secretion showed considerable variation, which, however, did not depend on the C-509T genotype (protein secretion: P=.879; gene expression: lymphocytes, P=.134, fibroblasts, P=.605). There was also no general correlation between TGFB1 expression and cellular radiosensitivity (lymphocytes, P=.632; fibroblasts, P=.573).
CONCLUSION: Our data indicate that any association between the SNP C-509T of TGFB1 and risk of normal tissue toxicity cannot be ascribed to a functional consequence of this SNP, either on the level of gene expression, protein secretion, or cellular radiosensitivity.
| Errataetall: |
CommentIn: Int J Radiat Oncol Biol Phys. 2013 Jul 1;86(3):400-1. - PMID 23708081 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:85 |
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| Enthalten in: |
International journal of radiation oncology, biology, physics - 85(2013), 2 vom: 01. Feb., Seite 460-5 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Reuther, Sebastian [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Completed 17.03.2013 Date Revised 11.07.2013 published: Print-Electronic CommentIn: Int J Radiat Oncol Biol Phys. 2013 Jul 1;86(3):400-1. - PMID 23708081 Citation Status MEDLINE |
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| doi: |
10.1016/j.ijrobp.2012.01.090 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM217847943 |
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| 100 | 1 | |a Reuther, Sebastian |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a No effect of the transforming growth factor β1 promoter polymorphism C-509T on TGFB1 gene expression, protein secretion, or cellular radiosensitivity |
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| 500 | |a Date Revised 11.07.2013 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a CommentIn: Int J Radiat Oncol Biol Phys. 2013 Jul 1;86(3):400-1. - PMID 23708081 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2013 Elsevier Inc. All rights reserved. | ||
| 520 | |a PURPOSE: To study whether the promoter polymorphism (C-509T) affects transforming growth factor β1 gene (TGFB1) expression, protein secretion, and/or cellular radiosensitivity for both human lymphocytes and fibroblasts | ||
| 520 | |a METHODS AND MATERIALS: Experiments were performed with lymphocytes taken either from 124 breast cancer patients or 59 pairs of normal monozygotic twins. We used 15 normal human primary fibroblast strains as controls. The C-509T genotype was determined by polymerase chain reaction-restriction fragment length polymorphism or TaqMan single nucleotide polymorphism (SNP) genotyping assay. The cellular radiosensitivity of lymphocytes was measured by G0/1 assay and that of fibroblasts by colony assay. The amount of extracellular TGFB1 protein was determined by enzyme-linked immunosorbent assay, and TGFB1 expression was assessed via microarray analysis or reverse transcription-polymerase chain reaction | ||
| 520 | |a RESULTS: The C-509T genotype was found not to be associated with cellular radiosensitivity, neither for lymphocytes (breast cancer patients, P=.811; healthy donors, P=.181) nor for fibroblasts (P=.589). Both TGFB1 expression and TGFB1 protein secretion showed considerable variation, which, however, did not depend on the C-509T genotype (protein secretion: P=.879; gene expression: lymphocytes, P=.134, fibroblasts, P=.605). There was also no general correlation between TGFB1 expression and cellular radiosensitivity (lymphocytes, P=.632; fibroblasts, P=.573) | ||
| 520 | |a CONCLUSION: Our data indicate that any association between the SNP C-509T of TGFB1 and risk of normal tissue toxicity cannot be ascribed to a functional consequence of this SNP, either on the level of gene expression, protein secretion, or cellular radiosensitivity | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Metzke, Elisabeth |e verfasserin |4 aut | |
| 700 | 1 | |a Bonin, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Petersen, Cordula |e verfasserin |4 aut | |
| 700 | 1 | |a Dikomey, Ekkehard |e verfasserin |4 aut | |
| 700 | 1 | |a Raabe, Annette |e verfasserin |4 aut | |
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