Evaluation of the efficiency and reactogenicity of emulsion-based adjuvant systems in the manufacture of polyclonal enteroviral diagnostic sera
Whether various adjuvants might be used in the manufacture of commercial enteroviral diagnostic sera (EDS) was studied. The following adjuvants: Ribi, SAF-1, and TiterMax were compared; vaseline-lanoline emulsion used to prepare EDSs, as well as modified Freund's complete adjuvant served as controls. Chinchilla rabbits were intramuscularly injected enterovirus antigens (enterovirus 70 and ECHO 2) together with the adjuvant emulsions. TiterMax showed the highest efficiency comparable with the activity of Freund's adjuvant. The activities of Ribi, SAF-1, and vaseline-lanoline emulsion were 3-4 times lower. The neutralizing activity of the sera obtained after 2-3 (TiterMax) or 4-5 (Ribi, SAF-1) immunizations was maximal. Further immunizations resulted in a reduction in the titers of neutralizing antibodies. TiterMax and vaseline-lanoline emulsion caused minimal complications at the site of inoculation whereas SAF-1 and Ribi gave rise to severer inflammatory responses.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2011 |
|---|---|
| Erschienen: |
2011 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:56 |
|---|---|
| Enthalten in: |
Voprosy virusologii - 56(2011), 2 vom: 30. März, Seite 41-6 |
| Sprache: |
Russisch |
|---|
| Beteiligte Personen: |
Kozlov, V G [VerfasserIn] |
|---|
| Anmerkungen: |
Date Completed 29.06.2011 Date Revised 09.11.2016 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM207969728 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM207969728 | ||
| 003 | DE-627 | ||
| 005 | 20231224003138.0 | ||
| 007 | tu | ||
| 008 | 231224s2011 xx ||||| 00| ||rus c | ||
| 028 | 5 | 2 | |a pubmed24n0693.xml |
| 035 | |a (DE-627)NLM207969728 | ||
| 035 | |a (NLM)21545042 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a rus | ||
| 100 | 1 | |a Kozlov, V G |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Evaluation of the efficiency and reactogenicity of emulsion-based adjuvant systems in the manufacture of polyclonal enteroviral diagnostic sera |
| 264 | 1 | |c 2011 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 29.06.2011 | ||
| 500 | |a Date Revised 09.11.2016 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Whether various adjuvants might be used in the manufacture of commercial enteroviral diagnostic sera (EDS) was studied. The following adjuvants: Ribi, SAF-1, and TiterMax were compared; vaseline-lanoline emulsion used to prepare EDSs, as well as modified Freund's complete adjuvant served as controls. Chinchilla rabbits were intramuscularly injected enterovirus antigens (enterovirus 70 and ECHO 2) together with the adjuvant emulsions. TiterMax showed the highest efficiency comparable with the activity of Freund's adjuvant. The activities of Ribi, SAF-1, and vaseline-lanoline emulsion were 3-4 times lower. The neutralizing activity of the sera obtained after 2-3 (TiterMax) or 4-5 (Ribi, SAF-1) immunizations was maximal. Further immunizations resulted in a reduction in the titers of neutralizing antibodies. TiterMax and vaseline-lanoline emulsion caused minimal complications at the site of inoculation whereas SAF-1 and Ribi gave rise to severer inflammatory responses | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Adjuvants, Immunologic |2 NLM | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a Antigens, Viral |2 NLM | |
| 650 | 7 | |a Emulsions |2 NLM | |
| 650 | 7 | |a Immune Sera |2 NLM | |
| 650 | 7 | |a Polysorbates |2 NLM | |
| 650 | 7 | |a Syntex adjuvant formulation |2 NLM | |
| 650 | 7 | |a TiterMax |2 NLM | |
| 650 | 7 | |a 145380-33-2 |2 NLM | |
| 650 | 7 | |a Acetylmuramyl-Alanyl-Isoglutamine |2 NLM | |
| 650 | 7 | |a 53678-77-6 |2 NLM | |
| 650 | 7 | |a Squalene |2 NLM | |
| 650 | 7 | |a 7QWM220FJH |2 NLM | |
| 650 | 7 | |a Poloxalene |2 NLM | |
| 650 | 7 | |a 9003-11-6 |2 NLM | |
| 650 | 7 | |a Freund's Adjuvant |2 NLM | |
| 650 | 7 | |a 9007-81-2 |2 NLM | |
| 700 | 1 | |a Viktorova, E G |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Voprosy virusologii |d 1956 |g 56(2011), 2 vom: 30. März, Seite 41-6 |w (DE-627)NLM000024201 |x 2411-2097 |7 nnns |
| 773 | 1 | 8 | |g volume:56 |g year:2011 |g number:2 |g day:30 |g month:03 |g pages:41-6 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 56 |j 2011 |e 2 |b 30 |c 03 |h 41-6 | ||