Optimization of lyophilization procedures for freeze-drying of human red blood cells

OBJECTIVE: To investigate the different parameters of the lyophilization procedures that affect the recovery of the rehydrated red blood cells (RBCs).

METHODS: Human RBCs loaded in tubes were cooled with 4 different modes and subjected to water bath at 25 degrees celsius;. The morphological changes of the RBCs were observed to assess the degree of vitrification, and the specimens were placed in the freeze-dryer with the temperature set up at 40, -50, -60, -70 and -80 degrees celsius;. The rates of temperature rise of the main and secondary drying in the lyophilization procedures were compared, and the water residue in the specimens was determined.

RESULTS: The protectant did not show ice crystal in the course of freezing and thawing. No significant difference was found in the recovery rate of the rehydrated RBCs freeze-dried at the minimum temperature of -70 degrees celsius; and -80 degrees celsius; (P > 0.05). The E procedure resulted in the maximum recovery of the RBCs (83.14% ± 9.55%) and Hb (85.33% ± 11.42%), showing significant differences from the other groups(P < 0.01 or 0.05). The recovery of the RBCs showed a positive correlation to the water residue in the samples.

CONCLUSION: Fast cooling in liquid nitrogen and shelf precooling at -70 degrees celsius; with a moderate rate of temperature rise in lyophylization and a start dry temperature close to the shelf equilibrium temperature produce optimal freeze-drying result of human RBCs.

Medienart:

Artikel

Erscheinungsjahr:

2010

Erschienen:

2010

Enthalten in:

Zur Gesamtaufnahme - volume:30

Enthalten in:

Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 30(2010), 9 vom: 15. Sept., Seite 2055-8

Sprache:

Chinesisch

Beteiligte Personen:

Chen, Lin-feng [VerfasserIn]
Liu, Jing-han [VerfasserIn]
Wang, De-qing [VerfasserIn]
Ouyang, Xi-lin [VerfasserIn]
Zhuang, Yuan [VerfasserIn]
Che, Ji [VerfasserIn]
Yu, Yang [VerfasserIn]
Li, Hui [VerfasserIn]

Themen:

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 28.11.2011

Date Revised 21.09.2010

published: Print

Citation Status MEDLINE

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM201700832