Optimization of lyophilization procedures for freeze-drying of human red blood cells
OBJECTIVE: To investigate the different parameters of the lyophilization procedures that affect the recovery of the rehydrated red blood cells (RBCs).
METHODS: Human RBCs loaded in tubes were cooled with 4 different modes and subjected to water bath at 25 degrees celsius;. The morphological changes of the RBCs were observed to assess the degree of vitrification, and the specimens were placed in the freeze-dryer with the temperature set up at 40, -50, -60, -70 and -80 degrees celsius;. The rates of temperature rise of the main and secondary drying in the lyophilization procedures were compared, and the water residue in the specimens was determined.
RESULTS: The protectant did not show ice crystal in the course of freezing and thawing. No significant difference was found in the recovery rate of the rehydrated RBCs freeze-dried at the minimum temperature of -70 degrees celsius; and -80 degrees celsius; (P > 0.05). The E procedure resulted in the maximum recovery of the RBCs (83.14% ± 9.55%) and Hb (85.33% ± 11.42%), showing significant differences from the other groups(P < 0.01 or 0.05). The recovery of the RBCs showed a positive correlation to the water residue in the samples.
CONCLUSION: Fast cooling in liquid nitrogen and shelf precooling at -70 degrees celsius; with a moderate rate of temperature rise in lyophylization and a start dry temperature close to the shelf equilibrium temperature produce optimal freeze-drying result of human RBCs.
Medienart: |
Artikel |
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Erscheinungsjahr: |
2010 |
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Erschienen: |
2010 |
Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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Enthalten in: |
Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 30(2010), 9 vom: 15. Sept., Seite 2055-8 |
Sprache: |
Chinesisch |
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Beteiligte Personen: |
Chen, Lin-feng [VerfasserIn] |
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Themen: |
Comparative Study |
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Anmerkungen: |
Date Completed 28.11.2011 Date Revised 21.09.2010 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM201700832 |
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---|---|---|---|
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100 | 1 | |a Chen, Lin-feng |e verfasserin |4 aut | |
245 | 1 | 0 | |a Optimization of lyophilization procedures for freeze-drying of human red blood cells |
264 | 1 | |c 2010 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 28.11.2011 | ||
500 | |a Date Revised 21.09.2010 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a OBJECTIVE: To investigate the different parameters of the lyophilization procedures that affect the recovery of the rehydrated red blood cells (RBCs) | ||
520 | |a METHODS: Human RBCs loaded in tubes were cooled with 4 different modes and subjected to water bath at 25 degrees celsius;. The morphological changes of the RBCs were observed to assess the degree of vitrification, and the specimens were placed in the freeze-dryer with the temperature set up at 40, -50, -60, -70 and -80 degrees celsius;. The rates of temperature rise of the main and secondary drying in the lyophilization procedures were compared, and the water residue in the specimens was determined | ||
520 | |a RESULTS: The protectant did not show ice crystal in the course of freezing and thawing. No significant difference was found in the recovery rate of the rehydrated RBCs freeze-dried at the minimum temperature of -70 degrees celsius; and -80 degrees celsius; (P > 0.05). The E procedure resulted in the maximum recovery of the RBCs (83.14% ± 9.55%) and Hb (85.33% ± 11.42%), showing significant differences from the other groups(P < 0.01 or 0.05). The recovery of the RBCs showed a positive correlation to the water residue in the samples | ||
520 | |a CONCLUSION: Fast cooling in liquid nitrogen and shelf precooling at -70 degrees celsius; with a moderate rate of temperature rise in lyophylization and a start dry temperature close to the shelf equilibrium temperature produce optimal freeze-drying result of human RBCs | ||
650 | 4 | |a Comparative Study | |
650 | 4 | |a English Abstract | |
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
700 | 1 | |a Liu, Jing-han |e verfasserin |4 aut | |
700 | 1 | |a Wang, De-qing |e verfasserin |4 aut | |
700 | 1 | |a Ouyang, Xi-lin |e verfasserin |4 aut | |
700 | 1 | |a Zhuang, Yuan |e verfasserin |4 aut | |
700 | 1 | |a Che, Ji |e verfasserin |4 aut | |
700 | 1 | |a Yu, Yang |e verfasserin |4 aut | |
700 | 1 | |a Li, Hui |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Nan fang yi ke da xue xue bao = Journal of Southern Medical University |d 2006 |g 30(2010), 9 vom: 15. Sept., Seite 2055-8 |w (DE-627)NLM16087646X |x 1673-4254 |7 nnns |
773 | 1 | 8 | |g volume:30 |g year:2010 |g number:9 |g day:15 |g month:09 |g pages:2055-8 |
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952 | |d 30 |j 2010 |e 9 |b 15 |c 09 |h 2055-8 |