Hepatitis C virus NS2/3 protease regulates HCV IRES-dependent translation and NS5B RdRp activity

Chronic hepatitis C virus (HCV) infection often leads to liver cancer. NS2/3 protease is the first of two virally encoded proteases required for HCV polyprotein processing. In this report, we investigated the function of NS2/3 protease on HCV replication and translation. Cells transfected with plasmids encoding wild-type or mutant NS2/3 and a dual-luciferase reporter construct containing an HCV internal ribosome entry site (IRES) were used to examine the effect of NS2/3 protease on translation of HCV RNA. Cells transfected with plasmids encoding wild-type or mutant NS2/3, pcDNA-NS5B and a reporter plasmid were used to examine the effect of NS2/3 protease on HCV replication. The results showed that both autocleavage processing and the uncleaved form of NS2/3 protease specifically decrease HCV IRES-directed translation, while the uncleaved form of NS2/3 protease decreases HCV NS5B RdRp activity (replication), indicating that autoregulation by NS2/3 protease of HCV replication and translation may play an important role in persistent HCV infection.

Medienart:

E-Artikel

Erscheinungsjahr:

2009

Erschienen:

2009

Enthalten in:

Zur Gesamtaufnahme - volume:154

Enthalten in:

Archives of virology - 154(2009), 9 vom: 18., Seite 1465-73

Sprache:

Englisch

Beteiligte Personen:

She, Yinglong [VerfasserIn]
Han, Tao [VerfasserIn]
Ye, Linbai [VerfasserIn]
Wu, Zhenghui [VerfasserIn]

Links:

Volltext

Themen:

Cysteine Endopeptidases
EC 2.7.7.48
EC 3.4.22.-
Journal Article
NS-5 protein, hepatitis C virus
NS2-3 protease
RNA, Viral
RNA-Dependent RNA Polymerase
Viral Nonstructural Proteins

Anmerkungen:

Date Completed 13.11.2009

Date Revised 10.01.2022

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1007/s00705-009-0469-7

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM190746688